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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Independent large scale duplications in multiple M. Tuberculosis lineages overlapping the same genomic region
PLoS ONE, Volume 7, No. 2, Article e26038, Year 2012
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Description
Mycobacterium tuberculosis, the causative agent of most human tuberculosis, infects one third of the world's population and kills an estimated 1.7 million people a year. With the world-wide emergence of drug resistance, and the finding of more functional genetic diversity than previously expected, there is a renewed interest in understanding the forces driving genome evolution of this important pathogen. Genetic diversity in M. tuberculosis is dominated by single nucleotide polymorphisms and small scale gene deletion, with little or no evidence for large scale genome rearrangements seen in other bacteria. Recently, a single report described a large scale genome duplication that was suggested to be specific to the Beijing lineage. We report here multiple independent large-scale duplications of the same genomic region of M. tuberculosis detected through whole-genome sequencing. The duplications occur in strains belonging to both M. tuberculosis lineage 2 and 4, and are thus not limited to Beijing strains. The duplications occur in both drug-resistant and drug susceptible strains. The duplicated regions also have substantially different boundaries in different strains, indicating different originating duplication events. We further identify a smaller segmental duplication of a different genomic region of a lab strain of H37Rv. The presence of multiple independent duplications of the same genomic region suggests either instability in this region, a selective advantage conferred by the duplication, or both. The identified duplications suggest that large-scale gene duplication may be more common in M. tuberculosis than previously considered. © 2012 Weiner et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s001.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s002.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s003.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s004.txt
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s005.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s006.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s007.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s008.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s009.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3274525/bin/pone.0026038.s010.tif
Authors & Co-Authors
Weiner, Brian K.
United States, Cambridge
Broad Institute
Gomez, James E.
United States, Cambridge
Broad Institute
Victor, Thomas Calldo
South Africa, Stellenbosch
Stellenbosch University
Warren, Robin Mark
South Africa, Stellenbosch
Stellenbosch University
Sloutsky, Alex
United States, Worcester
University of Massachusetts Chan Medical School
Plikaytis, Bonnie B.
United States, Atlanta
National Center for Hiv, Viral Hepatitis, Std, and tb Prevention
Posey, James E.
United States, Atlanta
National Center for Hiv, Viral Hepatitis, Std, and tb Prevention
Van Helden, Paul D.
South Africa, Stellenbosch
Stellenbosch University
Gey van Pittius, Nicolaas Claudius
South Africa, Stellenbosch
Stellenbosch University
Koehrsen, Michael
United States, Cambridge
Broad Institute
Sisk, Peter
United States, Cambridge
Broad Institute
Stolte, Christian
United States, Cambridge
Broad Institute
White, Jared R.
United States, Cambridge
Broad Institute
Gagneux, Sébastien P.
United Kingdom, London
Mrc National Institute for Medical Research
Birren, Bruce W.
United States, Cambridge
Broad Institute
Hung, Deborah T.
United States, Cambridge
Broad Institute
Murray, Megan B.
United States, Boston
Harvard T.h. Chan School of Public Health
Galagan, James E.
United States, Cambridge
Broad Institute
United States, Boston
Boston University
Statistics
Citations: 32
Authors: 18
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pone.0026038
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study