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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Effects of interleukin-10 on monocyte/endothelial cell adhesion and MMP-9/TIMP-1 secretion
Cardiovascular Research, Volume 49, No. 4, Year 2001
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Description
Objective: Monocyte adhesion to endothelial cells and subsequent secretion of matrix metalloproteinases (MMPs) by activated macrophages are key events in arteriosclerosis and restenosis. We tested the hypothesis that interleukin-10 (IL-10), a potent anti-inflammatory cytokine, inhibits monocyte-endothelial cell interactions. Methods: The effect of IL-10 on monocyte/endothelial cell adhesion, as well as on the expression of MMP-9 and the tissue inhibitor of MMP-9, TIMP-1, were first tested in vitro in coculture systems. In addition, we used an ex vivo binding assay to study the inhibitory effect of IL-10 on monocyte adhesion to carotid arteries obtained from either normal, or l-nitro arginine-methyl ester (l-NAME)-treated rats. The effect of IL-10 on the expression of monocyte adhesion molecules (CD18 and CD62-L) was studied by flow cytometry. Results: IL-10 (150 ng/ml) inhibits monocyte adhesion to endothelial cells (by 35%) and to carotid arteries (by 40 and 50%, in normal and l-NAME-treated rats, respectively), via direct modulation of the expression of CD18 and CD62-L. Moreover, IL-10 dose-dependently decreases MMP-9 activity and increases TIMP-1 levels in coculture systems, both at the transcriptional level. Conclusions: Our results suggest that IL-10 is an important modulator of monocyte-endothelial cell interactions. © 2001 Elsevier Science B.V.
Authors & Co-Authors
Mostafa Mtairag, El
France, Paris
Inserm
Morocco, Casablanca
Hassan Ii University of Casablanca
Chollet-Martin, Sylvie
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Oudghiri, Mounia
Morocco, Casablanca
Hassan Ii University of Casablanca
Laquay, Nathalie
France, Paris
Inserm
Jacob, Marie Paule
France, Paris
Inserm
Michel, Jean Baptiste L.
France, Paris
Inserm
Feldman, Laurent J.
France, Paris
Inserm
Statistics
Citations: 108
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/S0008-6363(00)00287-X
ISSN:
00086363