Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75
Journal of General Virology, Volume 85, No. 11, Year 2004
Notification
URL copied to clipboard!
Description
Sequencing of the gene that encodes the capsid protein VP1 has been used as a surrogate for antigenic typing in order to distinguish enterovirus serotypes; three new serotypes were identified recently by this method. In this study, 14 enterovirus isolates from six countries were characterized as members of two new types within the species Human enterovirus B, based on sequencing of the complete capsid-encoding (P1) region. Isolates within each of these two types differed significantly from one another and from all other known enterovirus serotypes on the basis of sequences that encode either VP1 alone or the entire P1 region. Members of each type were ≥77.2% identical to one another (89.5% amino acid identity) in VP1, but members of the two different types differed from one another and from other enteroviruses by ≥-31% in nucleotide sequence (25% amino acid sequence difference), indicating that the two groups represent separate new candidate enterovirus types. The complete P1 sequences differed from those of all other enterovirus serotypes by >1% (26% amino acid sequence difference), but were highly conserved within a serotype (≤8% amino acid sequence difference). Phylogenetic analyses demonstrated that isolates of the same serotype were monophyletic in both VP1 and the capsid as a whole, as shown previously for other enterovirus serotypes. This paper proposes that these 14 isolates should be classified as members of two new human enterovirus types, enteroviruses 74 and 75 (EV74 and EV75). © 2004 SGM.
Authors & Co-Authors
Oberste, Mark Steven
United States, Atlanta
Viral and Rickettsial Zoonoses Br.
Michele, Suzanne M.
United States, Atlanta
Viral and Rickettsial Zoonoses Br.
United States, Brisbane
Vaxgen, Inc.
Maher, Kaija
United States, Atlanta
Viral and Rickettsial Zoonoses Br.
Schnurr, David
United States, Berkeley
Viral Rickettsial Disease Laboratory
Cisterna, Daniel
Argentina, Buenos Aires
Administracion Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbran
Junttila, Nina
Sweden, Solna
Swedish Institute for Disease Control
Uddin, Moyez
Bangladesh, Dhaka
Institute of Public Health Dhaka
Chomel, Jean Jacques
France, Lyon
Ctr. Natl. Ref. Pour Les Enterovirus
Lau, Chi Shan
Hong Kong
Queen Mary Hospital Hong Kong
Ridha, Walid
Iraq, Baghdad
National Polio Laboratory
Al-Busaidy, S. M.
Oman, Muscat
Ministry of Health Oman
Norder, Heléne
Sweden, Solna
Swedish Institute for Disease Control
Magnius, Lars O.
Sweden, Solna
Swedish Institute for Disease Control
Pallansch, Mark A.
United States, Atlanta
Viral and Rickettsial Zoonoses Br.
Statistics
Citations: 127
Authors: 14
Affiliations: 10
Identifiers
Doi:
10.1099/vir.0.80148-0
ISSN:
00221317
Research Areas
Genetics And Genomics