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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Activated Rho/Rho kinase and modified calcium sensitivity in cryopreserved human saphenous veins
Cryobiology, Volume 57, No. 1, Year 2008
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Description
Background: We have shown previously that cryopreservation of human internal mammary arteries activates protein kinase C and enhances intracellular Ca2+ [Ca2+]i. We now present evidence that in human saphenous veins (HSV) cryoinjury is associated with activation of the Rho/Rho kinase signaling pathways and enhanced [Ca2+]i. Methods: HSV were investigated in vitro either unfrozen within 12 h after removal or after storage at -196 °C in a cryomedium containing 1.8 M dimethyl sulfoxide and 0.1 M sucrose as cryoprotectant additives. Results: Cryostorage diminished responses to receptor-mediated contractile agonists such as noradrenaline, 5-HT and endothelin-1 by up to 30% whereas responses to KCl were attenuated by about 50%. Concentration-response curves for CaCl2 on unfrozen and cryopreserved HSV revealed similar inhibitory activities of both blocking 1,4-dihydropyridine derivatives nifedipine and the (-)-(R) enantiomer of SDZ 202-791 whereas the Ca2+ channel activating (+)-(S) enantiomer of SDZ 202-791 was 10 times less effective at enhancing contractions to CaCl2 when tested after cryostorage. These functional effects were reflected by changes in [Ca2+]i as demonstrated by fluorescence of Fluo-3AM loaded veins. The diminished activity of (+)-(S) SDZ 202-791 in cryopreserved HSV was reversed partially when the potassium channel opener pinacidil (1 μM) was present during the freezing/thawing process. Blockade of Rho kinase by HA-1077 proved to be significantly more effective at attenuating contractile responses to both endothelin-1 and KCl after cryostorage. Conclusions: Data suggested that cryopreservation modified [Ca2+]i of venous smooth muscle cells (1) through depolarization-induced changes in Ca2+ influx and (2) through activation of Rho kinase signaling pathways. © 2008 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Müller-Schweinitzer, Else
Switzerland, Basel
Universitätsspital Basel
Reineke, David
Switzerland, Bern
Swiss Cardiovascular Center, University Hospital Bern
Glusa, Erika
Germany, Jena
Friedrich-schiller-universität Jena
Ebeigbe, Anthony B.
Nigeria, Benin
University of Benin
Grapow, Martin T.R.
Switzerland, Basel
Universitätsspital Basel
Carrel, Thierry Pierre
Switzerland, Basel
Universitätsspital Basel
Switzerland, Bern
Swiss Cardiovascular Center, University Hospital Bern
Statistics
Citations: 6
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/j.cryobiol.2008.05.003
e-ISSN:
10902392