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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
A constitutively active form of a durum wheat Na
+
/H
+
antiporter SOS1 confers high salt tolerance to transgenic Arabidopsis
Plant Cell Reports, Volume 33, No. 2, Year 2014
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Description
Key message: Expression of a truncated form of wheat TdSOS1 in Arabidopsis exhibited an improved salt tolerance. This finding provides new hints about this protein that can be considered as a salt tolerance determinant. The SOS signaling pathway has emerged as a key mechanism in preserving the homeostasis of Na+ and K+ under saline conditions. We have recently identified and functionally characterized, by complementation studies in yeast, the gene encoding the durum wheat plasma membrane Na+/H+ antiporter (TdSOS1). To extend these functional studies to the whole plant level, we complemented Arabidopsis sos1-1 mutant with wild-type TdSOS1 or with the hyperactive form TdSOS1{increment}972 and compared them to the Arabidopsis AtSOS1 protein. The Arabidopsis sos1-1 mutant is hypersensitive to both Na+ and Li+ ions. Compared with sos1-1 mutant transformed with the empty binary vector, seeds from TdSOS1 or TdSOS1{increment}972 transgenic plants had better germination under salt stress and more robust seedling growth in agar plates as well as in nutritive solution containing Na+ or Li+ salts. The root elongation of TdSOS1{increment}972 transgenic lines was higher than that of Arabidopsis sos1-1 mutant transformed with TdSOS1 or with the endogenous AtSOS1 gene. Under salt stress, TdSOS1{increment}972 transgenic lines showed greater water retention capacity and retained low Na+ and high K+ in their shoots and roots. Our data showed that the hyperactive form TdSOS1{increment}972 conferred a significant ionic stress tolerance to Arabidopsis plants and suggest that selection of hyperactive alleles of the SOS1 transport protein may pave the way for obtaining salt-tolerant crops. © 2013 Springer-Verlag Berlin Heidelberg.
Authors & Co-Authors
Feki, Kaouthar
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Quintero, Francisco J.
Spain, Sevilla
Csic - Instituto de Recursos Naturales y Agrobiologia de Sevilla Irnas
Khoudi, Habib
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Leidi, Eduardo O.
Spain, Sevilla
Csic - Instituto de Recursos Naturales y Agrobiologia de Sevilla Irnas
Masmoudi, Khaled
Tunisia, Sfax
Centre de Biotechnologie de Sfax
United Arab Emirates, Dubai
International Center for Biosaline Agriculture
Pardo, José Manuel
Spain, Sevilla
Csic - Instituto de Recursos Naturales y Agrobiologia de Sevilla Irnas
Brini, Faïçal
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Statistics
Citations: 96
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1007/s00299-013-1528-9
ISSN:
07217714
Research Areas
Environmental
Genetics And Genomics