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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Prevalence of antibiotic resistance genes in the bacterial flora of integrated fish farming environments of Pakistan and Tanzania
Environmental Science and Technology, Volume 46, No. 16, Year 2012
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Description
The use of a wide variety of antimicrobials in human and veterinary medicine, including aquaculture, has led to the emergence of antibiotic resistant pathogens. In the present study, bacteria from water, sediments, and fish were collected from fish farms in Pakistan and Tanzania with no recorded history of antibiotic use. The isolates were screened for the presence of resistance genes against various antimicrobials used in aquaculture and animal husbandry. Resistant isolates selected by disk diffusion and genotyped by Southern hybridization were further screened by polymerase chain reaction (PCR) and amplicon sequencing. The prominent resistance genes identified encoded tetracycline [tetA(A) and tetA(G)], trimethoprim [dfrA1, dfrA5, dfrA7, dfrA12, and dfrA15], amoxicillin [blaTEM], streptomycin [strA-strB], chloramphenicol [cat-1], and erythromycin resistance [mefA]. The int1 gene was found in more than 30% of the bacterial isolates in association with gene cassettes. MAR indices ranged from 0.2 to 1. The blaNDM-1 gene was not identified in ertapenem resistant isolates. It is hypothesized that integrated fish farming practices utilizing domestic farm and poultry waste along with antibiotic residues from animal husbandry may have contributed to a pool of resistance genes in the aquaculture systems studied. © 2012 American Chemical Society.
Authors & Co-Authors
Shah, Syed Q.A.
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Colquhoun, Duncan John
Norway, Oslo
Veterinærinstituttet
Nikuli, Hamisi L.
Tanzania, Dar es Salaam
Ministry of Livestock and Fisheries Development
Sorum, Henning
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Statistics
Citations: 164
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1021/es3018607
ISSN:
0013936X
e-ISSN:
15205851
Research Areas
Environmental
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study
Study Locations
Tanzania