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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Effect of herbal melanin on IL-8: A possible role of Toll-like receptor 4 (TLR4)
Biochemical and Biophysical Research Communications, Volume 344, No. 4, Year 2006
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Description
The production of IL-8 can be induced by LPS via TLR4 signaling pathway. In this study, we tested the effect of a herbal melanin (HM) extract, from black cumin seeds (Nigella sativa L.), on IL-8 production. We used HM and LPS in parallel to induce IL-8 production by THP-I, PBMCs, and TLR4-transfected HEK293 cells. Both HM and LPS induced IL-8 mRNA expression and protein production in THP-1 and PBMCs. On applying similar treatment to HEK293 cells that express TLR4, MD2, and CD14, both HM and LPS significantly induced IL-8 protein production. We have also demonstrated that HM and LPS had identical effects in terms of IL-8 stimulation by HEK293 transfected with either TLR4 or MD2-CD14. Melanin extracted from N. sativa L. mimics the action of LPS in the induction of IL-8 by PBMC and the other used cell lines. Our results suggest that HM may share a signaling pathway with LPS that involves TLR4. © 2006.
Authors & Co-Authors
El-Obeid, Adila Salih
Sweden, Uppsala
Akademiska Sjukhuset
Hassib, Adil M.
Saudi Arabia, Riyadh
King Saud University
Pontén, Fredrik C.
Sweden, Uppsala
Akademiska Sjukhuset
Westermark, Bengt
Sweden, Uppsala
Akademiska Sjukhuset
Statistics
Citations: 4
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.bbrc.2006.04.035
ISSN:
0006291X
e-ISSN:
10902104