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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Comparison of five in vitro bioassays to measure estrogenic activity in environmental waters
Environmental Science and Technology, Volume 44, No. 10, Year 2010
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Description
Bioassays are well established in the pharmaceutical industry and single compound analysis, but there is still uncertainty about their usefulness in environmental monitoring. We compared the responses of five bioassays designed to measure estrogenic activity (the yeast estrogen screen, ER-CALUX, MELN, T47D-KBluc, and E-SCREEN assays) and chemical analysis on extracts from four different water sources (groundwater, raw sewage, treated sewage, and river water). All five bioassays displayed similar trends and there was good agreement with analytical chemistry results. The data from the ER-CALUX and E-SCREEN bioassays were robust and predictable, and well-correlated with predictions from chemical analysis. The T47D-KBluc appeared likewise promising, but with a more limited sample size it was less compelling. The YES assay was less sensitive than the other assays by an order of magnitude, which resulted in a larger number of nondetects. The MELN assay was less predictable, although the possibility that this was due to laboratory-specific difficulties cannot be discounted. With standardized bioassay data analysis and consistency of operating protocols, bioanalytical tools are a promising advance in the development of a tiered approach to environmental water quality monitoring. © 2010 American Chemical Society.
Authors & Co-Authors
Leusch, Frederic D.L.
United Kingdom, London
Global Water Research Coalition
Australia, Brisbane
Griffith University
De Jager, Tiaan
South Africa, Pretoria
University of Pretoria
Levi, Yves
France, Gif-sur-yvette
Université Paris-saclay
Lim, Richard
Australia, Sydney
University of Technology Sydney
Puijker, Leo
Netherlands, Nieuwegein
Kwr Water Research Institute
Sacher, Frank
Germany, Karlsruhe
Dvgw - Technologiezentrum Wasser
Tremblay, Louis A.
New Zealand, Lincoln
Manaaki Whenua – Landcare Research
Wilson, Vickie S.
United States, Research Triangle Park
Us Epa National Health and Environmental Effects Research Laboratory
Chapman, Heather F.
Australia, Brisbane
Griffith University
Statistics
Citations: 197
Authors: 9
Affiliations: 9
Identifiers
Doi:
10.1021/es903899d
ISSN:
0013936X
e-ISSN:
15205851
Research Areas
Environmental