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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Invasion pathways and malaria severity in Kenyan Plasmodium falciparum clinical isolates
Infection and Immunity, Volume 75, No. 6, Year 2007
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Description
The invasion of erythrocytes byPlasmodium falciparum occurs through multiple pathways that can be studied in vitro by examining the invasion of erythrocytes treated with enzymes such as neuraminidase, trypsin, and chymotrypsin. We have studied the invasion pathways used by 31 Kenyan P. falciparum isolates from children with uncomplicated or severe malaria. Six distinct invasion profiles were detected, out of eight possible profiles. The majority of isolates (23 of 31) showed neuraminidase-resistant, trypsin-sensitive invasion, characteristic of the pathway mediated by an unknown parasite ligand and erythrocyte receptor "X." The neuraminidase-sensitive, trypsin-sensitive phenotype consistent with invasion mediated by the binding of parasite ligand erythrocyte binding antigen 175 to glycophorin A, the most common invasion profile in a previous study of Gambian field isolates, was seen in only 3 of 31 Kenyan isolates. No particular invasion profile was associated with severe P. falciparum malaria, and there was no significant difference in the levels of inhibition by the various enzyme treatments between isolates from children with severe malaria and those from children with uncomplicated malaria (P, >0.1 for all enzymes; Mann-Whitney U test). These results do not support the hypothesis that differences in invasion phenotypes play an important role in malaria virulence and indicate that considerable gaps remain in our knowledge of the molecular basis of invasion pathways in natural P. falciparum infections. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Deans, Anne Marie
United Kingdom, Edinburgh
The University of Edinburgh
Vaz Nery, Susana
United Kingdom, London
London School of Hygiene & Tropical Medicine
Conway, David J.
United Kingdom, London
London School of Hygiene & Tropical Medicine
Kai, Oscar K.
United States, Philadelphia
Glaxosmithkline, Usa
Marsh, Kevin
United States, Philadelphia
Glaxosmithkline, Usa
Rowe, Jane Alexandra
United Kingdom, Edinburgh
The University of Edinburgh
Statistics
Citations: 45
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1128/IAI.00249-07
ISSN:
00199567
Research Areas
Infectious Diseases
Maternal And Child Health