Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of antibodies against capripoxviruses using an inactivated sheeppox virus ELISA
Transboundary and Emerging Diseases, Volume 56, No. 4, Year 2009
Notification
URL copied to clipboard!
Description
An indirect ELISA was developed to detect antibodies specific for capripoxviruses in goat, sheep and cattle sera. Heat-inactivated Nigerian sheeppox virus was used as the ELISA antigen. Sera obtained from sheep and goats that were experimentally infected with different capripoxvirus isolates were used to develop and evaluate the sensitivity of the ELISA. Virus neutralization indexes were determined for the experimental sera in OA3.Ts cells. The specificity of the ELISA was determined using 231 sera from capripoxvirus naïve sheep and goats from Canada. In addition, the ELISA was tested for cross-reactivity to anti-orf virus antibodies using orf-reactive sera and no cross-reactivity was observed. Using experimentally generated sera obtained from animals infected with virulent sheeppox or goatpox virus isolates, the diagnostic sensitivity of the ELISA was 96% with a diagnostic specificity of 95%, where the diagnostic sensitivity of the virus neutralization assay was 96% with a diagnostic specificity of 100%. Further evaluation of this ELISA, using 276 cattle serum samples that were positive by virus neutralization assays, revealed a diagnostic sensitivity of 88% with a specificity of 97%. These results indicated that the inactivated capripoxvirus ELISA can detect capripoxvirus-specific antibodies in sheep, goats and cattle that have been infected with virulent capripoxvirus isolates. Non-virulent capripoxvirus isolates, in contrast, did not elicit positive (≥1.5 Log10 neutralization index) antibody responses. © 2009 Blackwell Verlag GmbH.
Authors & Co-Authors
Babiuk, Shawn L.
Canada, Winnipeg
National Centre for Foreign Animal Disease Ncfad
Canada, Winnipeg
University of Manitoba
Wallace, David Brian
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
South Africa, Pretoria
University of Pretoria
Smith, Shirley Jacqueline
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Bowden, Timothy R.
Australia, Geelong
Csiro Livestock Industries
Dalman, B.
Canada, Winnipeg
National Centre for Foreign Animal Disease Ncfad
Parkyn, G.
Canada, Winnipeg
National Centre for Foreign Animal Disease Ncfad
Copps, J.
Canada, Winnipeg
National Centre for Foreign Animal Disease Ncfad
Boyle, D. B.
Australia, Geelong
Csiro Livestock Industries
Statistics
Citations: 71
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1111/j.1865-1682.2009.01067.x
ISSN:
18651674
e-ISSN:
18651682