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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris
International Journal of Systematic and Evolutionary Microbiology, Volume 56, No. 11, Year 2006
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Description
The species Phaseolus vulgaris is a promiscuous legume nodulated by several species of the family Rhizobiaceae. During a study of rhizobia nodulating this legume in Portugal, we isolated several strains that nodulate P. vulgaris effectively and also Macroptilium atropurpureum and Leucaena leucocephala, but they form ineffective nodules in Medicago sativa. According to phylogenetic analysis of the 16S rRNA gene sequence, the strains from this study belong to the genus Rhizobium, with Rhizobium rhizogenes and Rhizobium tropici as the closest related species, with 99.9 and 99.2% similarity, respectively, between the type strains of these species and strain P1-7T. The nodD and nifH genes carried by strain P1-7T are phylogenetically related to those of other species nodulating Phaseolus. This strain does not carry virulence genes present in the type strain of R. rhizogenes, ATCC 11325T. Analysis of the recA and atpD genes confirms this phylogenetic arrangement, showing low similarity with respect to those of R. rhizogenes ATCC 11325T (91.9 and 94.1 % similarity, respectively) and R. tropici IIB CIAT 899T (90.6% and 91.8% similarity, respectively). The intergenic spacer (ITS) of the strains from this study is phylogenetically divergent from those of R. rhizogenes ATCC 11235T and R. tropici CIAT 899T, with 85.9 and 82.8 % similarity, respectively, with respect to strain P1-7T. The tRNA profile and two-primer random amplified polymorphic DNA pattern of strain P1-7T are also different from those of R. rhizogenes ATCC 11235T and R. tropici CIAT 899T. The strains isolated in this study can be also differentiated from R. rhizogenes and R. tropici by several phenotypic characteristics. The results of DNA-DNA hybridization showed means of 28 and 25% similarity between strain P1-7T and R. rhizogenes ATCC 11235T and R. tropici CIAT 899T, respectively. All these data showed that the strains isolated in this study belong to a novel species of the genus Rhizobium, for which we propose the name Rhizobium lusitanum sp. nov.; the type strain is P1-7T (=LMG 22705T = CECT 7016T). © 2006 IUMS.
Authors & Co-Authors
Valverde, Ángel
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Israel, Jerusalem
Hebrew University of Jerusalem
Igual, J. M.
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Peix, Álvaro
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Cervantes, E.
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Velázquez, Encarna
Spain, Salamanca
Universidad de Salamanca
Statistics
Citations: 115
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1099/ijs.0.64402-0
ISSN:
14665026
Research Areas
Genetics And Genomics