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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia
Lipids in Health and Disease, Volume 10, Article 161, Year 2011
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Description
Background: In order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of M. vulgare L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines. Methods. The agar disk diffusion method was used to study the antibacterial activity of M. vulgare essential oil against 12 bacterial and 4 fungi strains. The disc diameters of zone of inhibition (DD), the minimum inhibitory concentrations (MIC) and the concentration inhibiting 50% (IC 50) were investigated to characterize the antimicrobial activities of this essential oil. The in vitro cytotoxicity of M. vulgare essential oil was examined using a modified MTT assay; the viability and the IC50were used to evaluate this test. Results: The antimicrobial activity of the essential oil was investigated in order to evaluate its efficacy against the different tested microorganisms. The present results results showed a significant activity against microorganisms especially Gram (+) bacteria with inhibition zones and minimal inhibitory concentration values in the range of 6.6-25.2 mm and 1120-2600 g/ml, respectively, whereas Gram (-) bacteria exhibited a higher resistance. As far as the antifungal activity, among four strains tested, Botrytis cinerea exhibited the strongest activity with inhibition zones of 12.6 mm. However, Fusarium solani, Penicillium digitatum and Aspergillus niger were less sensitive to M. vulgare essential oil. About the citotoxicity assay, this finding indicate the capability of this essential oil to inhibited the proliferation of HeLa cell lines under some conditions with IC50value of 0.258 g/ml. Conclusion: This investigation showed that the M. vulgare essential oil has a potent antimicrobial activity against some Gram (+) pathogenic bacteria and Botrytis cinerea fungi. The present studies confirm the use of this essential oil as anticancer agent. Further research is required to evaluate the practical values of therapeutic applications. © 2011 Zarai et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Zarai, Zied
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Kadri, Adel
Tunisia, Sfax
University of Sfax
Ben Chobba, Ines
Tunisia, Sfax
University of Sfax
Ben Mansour, Riadh
Tunisia, Sfax
Higher Institute of Biotechnology of Sfax Isbs
Békir, Ahmed
Tunisia, Sfax
University of Sfax
Mejdoub, Hafedh
Tunisia, Sfax
University of Sfax
Gharsallah, Néji
Tunisia, Sfax
University of Sfax
Statistics
Citations: 135
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1186/1476-511X-10-161
e-ISSN:
1476511X
Study Locations
Niger
Tunisia