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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
AFLP fingerprinting for the analysis of genetic diversity within Necator americanus
Molecular and Cellular Probes, Volume 20, No. 5, Year 2006
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Description
In the present study, we utilised the method of AFLP to screen for genetic variation within and among individuals of the blood-feeding human hookworm Necator americanus (Nematoda) from Africa, Asia and South America. A total of 45 adult worms (i.e. 20 from Ghana, 16 from Colombia and 9 from Nepal) were subjected to analysis using the restriction enzyme/primer combination HindIII+AG/BglII+AC. Cluster analysis divided N. americanus into multiple, genetically distinct groups, consistent with previous findings using ribosomal and mitochondrial DNA data sets. The results demonstrated the usefulness of AFLP fingerprinting for establishing genetic variation within N. americanus and reinforce its applicability to other parasitic helminths of human and/or veterinary health importance. © 2006 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
De Gruijter, J. M.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Polderman, Anton M.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Dijkshoorn, Lenie
Netherlands, Leiden
Leids Universitair Medisch Centrum
Roberts, Helen
Australia, Melbourne
University of Melbourne
Ziem, Juventus Benogle
Ghana
Parasitic Dis. Research Laboratory
Kunwar, Chhatra B.
India, Jaipur
University of Rajasthan
Gasser, Robin B.
Australia, Melbourne
University of Melbourne
Statistics
Citations: 7
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1016/j.mcp.2006.03.004
ISSN:
08908508
Research Areas
Genetics And Genomics
Study Locations
Ghana