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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Unfolding of a branched double-helical DNA three-way junction with triple-helical ends
Archives of Biochemistry and Biophysics, Volume 313, No. 1, Year 1994
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Description
We have designed three oligonucleotides (33 mers) which when mixed in a 1:1:1 ratio form double-helical DNA three-way junctions with triple helical ends in the pH interval pH 4 to 5.5. The triplex to coil transition is initiated by raising the temperature and was recorded by temperature gradient gel electrophoresis, uv melting, and differential scanning calorimetry. The transitions can be deconvoluted into three subtransitions representing the independent thermal denaturation of each of the arms. We have proposed a model for the unfolding pathway and give the thermodynamic parameters for each step as calculated using the formalism outlined in the appendix. © 1994 Academic Press, Inc.
Authors & Co-Authors
Hüsler, Paul L.
South Africa, Cape Town
University of Cape Town
Klump, Horst H.
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 2
Authors: 2
Affiliations: 1
Identifiers
Doi:
10.1006/abbi.1994.1354
ISSN:
00039861
Research Areas
Genetics And Genomics