Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Reduced artemisinin susceptibility of plasmodium falciparum ring stages in western cambodia
Antimicrobial Agents and Chemotherapy, Volume 57, No. 2, Year 2013
Notification
URL copied to clipboard!
Description
The declining efficacy of artemisinin derivatives against Plasmodium falciparum in western Cambodia is a major concern. The knowledge gap in the understanding of the mechanisms involved hampers designing monitoring tools. Here, we culture-adapted 20 isolates from Pailin and Ratanakiri (areas of artemisinin resistance and susceptibility in western and eastern Cambodia, respectively) and studied their in vitro response to dihydroartemisinin. No significant difference between the two sets of isolates was observed in the classical isotopic test. However, a 6-h pulse exposure to 700 nM dihydroartemisinin (ring-stage survival assay -RSA]) revealed a clear-cut geographic dichotomy. The survival rate of exposed ring-stage parasites (ring stages) was 17-fold higher in isolates from Pailin (median, 13.5%) than in those from Ratanakiri (median, 0.8%), while exposed mature stages were equally and highly susceptible (0.6% and 0.7%, respectively). Ring stages survived drug exposure by cell cycle arrest and resumed growth upon drug withdrawal. The reduced susceptibility to artemisinin in Pailin appears to be associated with an altered in vitro phenotype of ring stages from Pailin in the RSA. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Witkowski, Benoît
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Khim, Nimol
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Cambodia, Phnom Penh
Ministry of Health Cambodia
Chim, Pheaktra
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Kim, Saorin
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Cambodia, Phnom Penh
Ministry of Health Cambodia
Switzerland, Geneva
Organisation Mondiale de la Santé
Ke, Sopheakvatey
Thailand, Nakhon Pathom
Mahidol University
Chy, Sophy
Cambodia, Phnom Penh
Ministry of Health Cambodia
Socheat, Duong
Cambodia, Phnom Penh
Ministry of Health Cambodia
Leang, Rithea
Switzerland, Geneva
Organisation Mondiale de la Santé
Ringwald, Pascal
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Dondorp, A. M.
Cambodia, Phnom Penh
Ministry of Health Cambodia
Tripura, Rupam
Switzerland, Geneva
Organisation Mondiale de la Santé
Benoit-Vical, Françoise
Thailand, Nakhon Pathom
Mahidol University
Berry, Antoine
France, Toulouse
Chu de Toulouse
Ariey, Frédéric
France, Toulouse
Chu de Toulouse
Barale, Jean Christophe
France, Paris
Institut Pasteur, Paris
Mercereau-Puijalon, Odile
France, Toulouse
Chu de Toulouse
Menard, Didier
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Statistics
Citations: 195
Authors: 17
Affiliations: 6
Identifiers
Doi:
10.1128/AAC.01868-12
ISSN:
10986596