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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Involvement of cathepsin B in the plant disease resistance hypersensitive response
Plant Journal, Volume 52, No. 1, Year 2007
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Description
A diverse range of plant proteases are implicated in pathogen perception and in subsequent signalling and execution of disease resistance. We demonstrate, using protease inhibitors and virus-induced gene silencing (VIGS), that the plant papain cysteine protease cathepsin B is required for the disease resistance hypersensitive response (HR). VIGS of cathepsin B prevented programmed cell death (PCD) and compromised disease resistance induced by two distinct non-host bacterial pathogens. It also suppressed the HR triggered by transient co-expression of potato R3a and Phytophthora infestans Avr3a genes. However, VIGS of cathepsin B did not compromise HR following recognition of Cladosporium fulvum AVR4 by tomato Cf-4, indicating that plant PCD can be independent of cathepsin B. The non-host HR to Erwinia amylovora was accompanied by a transient increase in cathepsin B transcript level and enzymatic activity and induction of the HR marker gene Hsr203. VIGS of cathepsin B significantly reduced the induction of Hsr203 following E. amylovora challenge, further demonstrating a role for this protease in PCD. Whereas cathepsin B is often relocalized from the lysosome to the cytosol during animal PCD, plant cathepsin B is secreted into the apoplast, and is activated upon secretion in the absence of pathogen challenge. © 2007 The Authors.
Authors & Co-Authors
Gilroy, Eleanor M.
United Kingdom, Aberdeen
The James Hutton Institute
Hein, Ingo
United Kingdom, Aberdeen
The James Hutton Institute
Van Der Hoorn, Renier
Germany, Koln
Max Planck Institute for Plant Breeding Research
Boevink, Petra C.
United Kingdom, Aberdeen
The James Hutton Institute
Venter, Eduard
South Africa, Johannesburg
University of Johannesburg
United Kingdom, Aberdeen
The James Hutton Institute
McLellan, Hazel
United Kingdom, Aberdeen
The James Hutton Institute
United Kingdom, Edinburgh
The University of Edinburgh
Kaffarnik, Florian
United Kingdom, Norwich
The Sainsbury Laboratory
Hrubikova, Katarina
United Kingdom, Aberdeen
The James Hutton Institute
Shaw, Jane
United Kingdom, Aberdeen
The James Hutton Institute
Holeva, Maria
United Kingdom, Aberdeen
The James Hutton Institute
López, Eduardo C.
Cuba, Havana
Center for Genetic Engineering and Biotechnology Havana
Borras-Hidalgo, Orlando
Cuba, Havana
Center for Genetic Engineering and Biotechnology Havana
Pritchard, Leighton
United Kingdom, Aberdeen
The James Hutton Institute
Loake, Gary J.
United Kingdom, Edinburgh
The University of Edinburgh
Lacomme, Christophe
United Kingdom, Aberdeen
The James Hutton Institute
United Kingdom, Edinburgh
The University of Edinburgh
Birch, Paul R.J.
United Kingdom, Aberdeen
The James Hutton Institute
Statistics
Citations: 163
Authors: 16
Affiliations: 6
Identifiers
Doi:
10.1111/j.1365-313X.2007.03226.x
ISSN:
09607412
e-ISSN:
1365313X
Research Areas
Genetics And Genomics