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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Comparison of the Performances of Five Primer Sets for the Detection and Quantification of Plasmodium in Anopheline Vectors by Real-Time PCR
PLoS ONE, Volume 11, No. 7, Article e0159160, Year 2016
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Description
Quantitative real-time polymerase chain reaction (qrtPCR) has made a significant improvement for the detection of Plasmodium in anopheline vectors. A wide variety of primers has been used in different assays, mostly adapted from molecular diagnosis of malaria in human. However, such an adaptation can impact the sensitivity of the PCR. Therefore we compared the sensitivity of five primer sets with different molecular targets on blood stages, sporozoites and oocysts standards of Plasmodium falciparum (Pf) and P. vivax (Pv). Dilution series of standard DNA were used to discriminate between methods at low concentrations of parasite and to generate standard curves suitable for the absolute quantification of Plasmodium sporozoites. Our results showed that the best primers to detect blood stages were not necessarily the best ones to detect sporozoites. Absolute detection threshold of our qrtPCR assay varied between 3.6 and 360 Pv sporozoites and between 6 and 600 Pf sporozoites per mosquito according to the primer set used in the reaction mix. In this paper, we discuss the general performance of each primer set and highlight the need to use efficient detection methods for transmission studies. © 2016 Chaumeau et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4956213/bin/pone.0159160.s001.docx
Authors & Co-Authors
Andolina, Chiara
Thailand, Nakhon Pathom
Mahidol University
Tuikue-Ndam, Nicaise
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Communauté D'universités et D'etablissements Sorbonne Paris Cité
Brengues, Cécile
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Herder, S.
France, Montpellier
Interactions Hôtes-vecteurs-parasites-environnement Dans Les Maladies Tropicales Négligées Dues Aux Trypanosomatides Intertryp
Thailand, Bangkok
Kasetsart University
Chareonviriyaphap, Theeraphap
Thailand, Bangkok
Kasetsart University
Nosten, François Henry
Thailand, Nakhon Pathom
Mahidol University
United Kingdom, Oxford
University of Oxford
Corbel, Vincent
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Thailand, Bangkok
Kasetsart University
Statistics
Citations: 20
Authors: 7
Affiliations: 8
Identifiers
Doi:
10.1371/journal.pone.0159160
ISSN:
19326203
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Approach
Quantitative