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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Mono-and Polyassociation Processes of Pentavalent Biotinylated PEI Glycopolymers for the Fabrication of Biohybrid Structures with Targeting Properties
Biomacromolecules, Volume 20, No. 9, Year 2019
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Description
This study describes new mechanistic insights in the sequential polyassociation of streptavidin with biotinylated poly(ethyleneimine) glycopolymers and biotinylated PEGylated folic acid components for the preparation of biohybrid structures (BHS) for controlled targeting experiments. Characterization of the BHS revealed that during the formation and postfunctionalization of BHS, reversible dissociation and reassociation processes occur. The BHS are stable over weeks after finalizing the equilibrium-driven polyassociation process. Cellular uptake studies showed that this sequential polyassociation involving biotinylated PEGylated folic acid components does not lead to enhanced cellular uptake of the resulting BHS. In contrast, polyplexes, containing small interfering RNA and bioconjugates (1:1 molar ratio between biotinylated glycopolymer and monomeric streptavidin-lectin fusion protein), enabled us to control the targeting of tumor cells as revealed by knockdown of the tumor-associated protein survivin. Overall, this study demonstrates the high potential of (networklike) streptavidin-biotin interactions with a dynamic character in the formation of complex BHS and extracellular matrix materials. Copyright © 2019 American Chemical Society.
Authors & Co-Authors
Boye, Susanne
Germany, Dresden
Leibniz-institut Für Polymerforschung Dresden E.v.
Moreno, Silvia
Germany, Dresden
Leibniz-institut Für Polymerforschung Dresden E.v.
Lederer, Albena
Germany, Dresden
Leibniz-institut Für Polymerforschung Dresden E.v.
Germany, Dresden
Technische Universität Dresden
Temme, Achim
Germany, Dresden
Technische Universität Dresden
Germany, Heidelberg
German Cancer Research Center
Germany, Dresden
Nationales Centrum Für Tumorerkrankungen Dresden
Voit, Brigitte I.
Germany, Dresden
Leibniz-institut Für Polymerforschung Dresden E.v.
Germany, Dresden
Technische Universität Dresden
Appelhans, Dietmar
Germany, Dresden
Leibniz-institut Für Polymerforschung Dresden E.v.
Statistics
Citations: 6
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1021/acs.biomac.9b00667
ISSN:
15257797
Research Areas
Cancer