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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Antigenic and genetic analysis of H5 influenza viruses isolated from water birds for the purpose of vaccine use
Archives of Virology, Volume 153, No. 11, Year 2008
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Description
In order to prepare H5N1 influenza virus vaccine, the hemagglutinins (HAs) of 14 H5 virus isolates from water birds in Asia were antigenically and genetically analyzed. Phylogenetic analysis of the H5 HA genes revealed that 13 isolates belong to Eurasian and the other one to North American lineages. Each of the deduced amino acid sequences of the HAs indicated a non-pathogenic profile. Antigenic analysis using a panel of monoclonal antibodies recognizing six different epitopes on the HA of A/duck/Pennsylvania/10218/1984 (H5N2) and chicken antiserum to an H5N1 reassortant strain generated between A/duck/Mongolia/54/2001 (H5N2) and A/duck/Mongolia/47/2001 (H7N1), [R(Dk/Mong-Dk/Mong) (H5N1)] showed that the HAs of highly pathogenic avian influenza (HPAI) viruses currently circulating in Asia were antigenically closely related to those of the present isolates from water birds. Mice subcutaneously injected with formalin-inactivated R(Dk/Mong-Dk/Mong) were protected from challenge with 100 mouse lethal dose of A/Viet Nam/1194/2004 (H5N1). The present results support the notion that the H5 isolates and the reassortant H5N1 strain should be useful for vaccine preparation. © 2008 Springer-Verlag.
Authors & Co-Authors
Soda, Kosuke
Japan, Sapporo
Hokkaido University
Ozaki, Hiroichi
Japan, Tottori
Faculty of Agriculture, Tottori University
Sakoda, Yoshihiro
Japan, Sapporo
Hokkaido University
Isoda, Norikazu
Japan, Sapporo
Hokkaido University
Sakabe, Saori
Japan, Sapporo
Hokkaido University
Kuboki, Noritaka
Japan, Sapporo
Hokkaido University
Kishida, Noriko
Japan, Sapporo
Hokkaido University
Takada, Ayato
Japan, Sapporo
Hokkaido University
Kida, Hiroshi
Japan, Sapporo
Hokkaido University
Statistics
Citations: 30
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1007/s00705-008-0226-3
ISSN:
03048608
Research Areas
Environmental
Genetics And Genomics