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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its complex with the inhibitor acifluorfen
Journal of Biological Chemistry, Volume 281, No. 50, Year 2006
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Description
Protoporphyrinogen IX oxidase, a monotopic membrane protein, which catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX in the heme/chlorophyll biosynthetic pathway, is distributed widely throughout nature. Here we present the structure of protoporphyrinogen IX oxidase from Myxococcus xanthus, an enzyme with similar catalytic properties to human protoporphyrinogen IX oxidase that also binds the common plant herbicide, acifluorfen. In the native structure, the planar porphyrinogen substrate is mimicked by a Tween 20 molecule, tracing three sides of the macrocycle. In contrast, acifluorfen does not mimic the planarity of the substrate but is accommodated by the shape of the binding pocket and held in place by electrostatic and aromatic interactions. A hydrophobic patch surrounded by positively charged residues suggests the position of the membrane anchor, differing from the one proposed for the tobacco mitochondrial protoporphyrinogen oxidase. Interestingly, there is a discrepancy between the dimerization state of the protein in solution and in the crystal. Conserved structural features are discussed in relation to a number of South African variegate porphyria-causing mutations in the human enzyme. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
Authors & Co-Authors
Corradi, Hazel Ruth
United Kingdom, Bath
University of Bath
Corrigall, Anne V.
South Africa, Cape Town
University of Cape Town
Boix, Ester
United Kingdom, Bath
University of Bath
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Mohan, C. Gopi
United Kingdom, Bath
University of Bath
India, Mohali
National Institute of Pharmaceutical Education and Research, Mohali
Sturrock, Edward D.
South Africa, Cape Town
Faculty of Health Sciences
Meissner, Peter N.
South Africa, Cape Town
University of Cape Town
South Africa, Cape Town
Faculty of Health Sciences
Ravi Acharya, K.
United Kingdom, Bath
University of Bath
Statistics
Citations: 60
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1074/jbc.M606640200
ISSN:
00219258
e-ISSN:
1083351X
Research Areas
Substance Abuse