Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Mutations in the regulatory network underlie the recent clonal expansion of a dominant subclone of the Mycobacterium tuberculosis Beijing genotype
Infection, Genetics and Evolution, Volume 11, No. 3, Year 2011
Notification
URL copied to clipboard!
Description
The Beijing genotype family is an epidemiologically important sub-group of Mycobacterium tuberculosis. It has been suggested that the high frequency of the Beijing isolates in some areas could be explained by selective advantages. Some evidence suggests that the emerging and most frequently isolated " Typical Beijing" lineage has the ability to circumvent BCG-induced immunity.To investigate the phylogeny of the Beijing genotype of M. tuberculosis, the genome of six Beijing strains from three different countries was sequenced with next-generation sequencing. The phylogeny of these strains was established using single nucleotide polymorphisms (SNPs). The three Typical Beijing strains clustered very tightly in the Beijing phylogeny suggesting that Typical Beijing strains represent a monophyletic lineage and resulted from recent diversification. Typing of 150 M. tuberculosis strains with a subset of the SNPs and comparison of the IS. 6110 restriction-fragment length polymorphism (RFLP) patterns of these strains to a database of 1522 Beijing RFLP patterns revealed that about 80% of all Beijing strains belong to the Typical Beijing subclone, which indicates clonal expansion. To identify the genomic changes that are characteristic for all Typical Beijing strains and to reconstruct their most recent common ancestor, the presence of SNPs was assayed in other Beijing strains.We identified 51 SNPs that define the minimal set of polymorphisms for all Typical Beijing strains. Nonsynonymous polymorphisms in genes coding for the regulatory network were over-represented in this set of mutations. We suggest that alterations in the response to environmental signals may have enabled Typical Beijing strains to develop the emerging phenotype. © 2011 Elsevier B.V.
Authors & Co-Authors
Schürch, Anita Christina
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Netherlands, Nijmegen
Radboud University Medical Center
Kremer, Kristin
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Warren, Robin Mark
South Africa, Stellenbosch
Stellenbosch University
Hung, Nguyen V.
Viet Nam, Hanoi
National Lung Hospital
Zhao, Yanlin
China, Hong Kong
Hong Kong Government
Wan, Kanglin
China, Beijing
Chinese Center for Disease Control and Prevention
Boeree, Martin Johan
Netherlands, Nijmegen
Radboud University Medical Center
Siezen, Roland J.
Netherlands, Nijmegen
Radboud University Medical Center
Smith, Noel H.
United Kingdom, Addlestone
Animal and Plant Health Agency
United Kingdom, Brighton
University of Sussex
van Soolingen, Dick
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Netherlands, Nijmegen
Radboud University Medical Center
Statistics
Citations: 43
Authors: 10
Affiliations: 8
Identifiers
Doi:
10.1016/j.meegid.2011.01.009
ISSN:
15671348
Research Areas
Genetics And Genomics