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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Profiling humoral immune responses to P. falciparum infection with protein microarrays
Proteomics, Volume 8, No. 22, Year 2008
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Description
A complete description of the serological response following exposure of humans to complex pathogens is lacking and approaches suitable for accomplishing this are limited. Here we report, using malaria as a model, a method which elucidates the profile of antibodies that develop after natural or experimental infection or after vaccination with attenuated organisms, and which identifies immunoreactive antigens of interest for vaccine development or other applications. Expression vectors encoding 250 Plasmodium falciparum (Pf) proteins were generated by PCR/recombination cloning; the proteins were individually expressed with >90% efficiency in Escherichia coli cell-free in vitro transcription and translation reactions, and printed directly without purification onto microarray slides. The protein microarrays were probed with human sera from one of four groups which differed in immune status: sterile immunity or no immunity against experimental challenge following vaccination with radiation-attenuated Pf sporozoites, partial immunity acquired by natural exposure, and no previous exposure to Pf. Overall, 72 highly reactive Pf antigens were identified. Proteomic features associated with immunoreactivity were identified. Importantly, antibody profiles were distinct for each donor group. Information obtained from such analyses will facilitate identifying antigens for vaccine development, dissecting the molecular basis of immunity, monitoring the outcome of whole-organism vaccine trials, and identifying immune correlates of protection. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
Authors & Co-Authors
Doolan, Denise L.
United States, Silver Spring
Naval Medical Research Center
United States, Baltimore
Johns Hopkins Bloomberg School of Public Health
Australia, Brisbane
Qimr Berghofer Medical Research Institute
Mu, Yunxiang
United States, Irvine
Uci School of Medicine
Unal, Berkay
United States, Irvine
Uci School of Medicine
Sundaresh, Suman
United States, Irvine
University of California, Irvine
Hirst, Siddiqua
United States, Irvine
Uci School of Medicine
Valdez, Conrad
United States, Irvine
Uci School of Medicine
Randall, Arlo
United States, Irvine
University of California, Irvine
Molina, Douglas M.
United States, Irvine
Immport Therapeutics Inc.
Liang, Xiaowu
United States, Irvine
Immport Therapeutics Inc.
Freilich, Daniel A.
United States, Silver Spring
Naval Medical Research Center
Oloo, James Aggrey
Kenya, Nairobi
Kenya Medical Research Institute
Tanzania, Dar es Salaam
African Malaria Network Trust Amanet
Blair, Peter L.
United States, Silver Spring
Naval Medical Research Center
United States, Richmond
Earlham College
Aguiar, João Carlos
United States, Silver Spring
Naval Medical Research Center
Baldi, Pierre F.
United States, Irvine
University of California, Irvine
Davies, David Huw
United States, Irvine
Uci School of Medicine
United States, Irvine
University of California, Irvine
Felgner, Philip Louis
United States, Irvine
Uci School of Medicine
United States, Irvine
University of California, Irvine
Statistics
Citations: 268
Authors: 16
Affiliations: 9
Identifiers
Doi:
10.1002/pmic.200800194
ISSN:
16159853
e-ISSN:
16159861
Research Areas
Cancer
Infectious Diseases