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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Evaluation and selection of tandem repeat loci for Streptococcus pneumoniae MLVA strain typing
BMC Microbiology, Volume 5, Article 66, Year 2005
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Description
Background: Precise identification of bacterial pathogens at the strain level is essential for epidemiological purposes. In Streptococcus pneumoniae, the existence of 90 different serotypes makes the typing particularly difficult and requires the use of highly informative tools. Available methods are relatively expensive and cannot be used for large-scale or routine typing of any new isolate. We explore here the potential of MLVA (Multiple Loci VNTR Analysis; VNTR, Variable Number of Tandem Repeats), a method of growing importance in the field of molecular epidemiology, for genotyping of Streptococcus pneumoniae. Results: Available genome sequences were searched for polymorphic tandem repeats. The loci identified were typed across a collection of 56 diverse isolates and including a group of serotype 1 isolates from Africa. Eventually a set of 16 VNTRs was proposed for MLVA-typing of S. pneumoniae. These robust markers were sufficient to discriminate 49 genotypes and to aggregate strains on the basis of the serotype and geographical origin, although some exceptions were found. Such exceptions may reflect serotype switching or horizontal transfer of genetic material. Conclusion: We describe a simple PCR-based MLVA genotyping scheme for S. pneumoniae which may prove to be a powerful complement to existing tools for epidemiological studies. Using this technique we uncovered a clonal population of strains, responsible for infections in Burkina Faso. We believe that the proposed MLVA typing scheme can become a standard for epidemiological studies of S. pneumoniae. © 2005 Koeck et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Koeck, Jean Louis
France, Villenave-d'ornon
Hôpital D'instruction Des Armées Robert Picqué
Njanpop-Lafourcade, Berthe Marie
France, Paris
Association Pour L'aide à la Médecine Préventive
Cade, Sonia
France, Villenave-d'ornon
Hôpital D'instruction Des Armées Robert Picqué
Varon, Emmanuelle
France, Paris
Hôpital Européen Georges-pompidou
Sangaré, Lassana
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Valjevac, Samina
France, Gif-sur-yvette
Université Paris-saclay
France, Vert-le-petit
Centre D'études du Bouchet
Vergnaud, Gilles
France, Gif-sur-yvette
Université Paris-saclay
France, Vert-le-petit
Centre D'études du Bouchet
Pourcel, Christine
France, Gif-sur-yvette
Université Paris-saclay
Statistics
Citations: 8
Authors: 8
Affiliations: 6
Identifiers
Doi:
10.1186/1471-2180-5-66
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Burkina Faso