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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Effects of fermented rooibos (Aspalathus linearis) on adipocyte differentiation
Phytomedicine, Volume 21, No. 2, Year 2014
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Description
Rooibos (Aspalathus linearis) contains a rich complement of polyphenols, including flavonoids, considered to be largely responsible for its health promoting effects, including combatting obesity. The purpose of this study was to examine the effect of fermented rooibos hot water soluble solids on in vitro adipocyte differentiation by using differentiating 3T3-L1 adipocytes. Hot water soluble solids were obtained when preparing an infusion of fermented rooibos at "cup-of-tea" strength. The major phenolic compounds (>5 mg/g) were isoorientin, orientin, quercetin-3-O-robinobioside and enolic phenylpyruvic acid-2-O-β-d-glucoside. Treatment of 3T3-L1 adipocytes with 10 μg/ml and 100 μg/ml of the rooibos soluble solids inhibited intracellular lipid accumulation by 22% (p < 0.01) and 15% (p < 0.05), respectively. Inhibition of adipogenesis was accompanied by decreased messenger RNA (mRNA) expression of PPARγ, PPARα, SREBF1 and FASN. Western blot analysis exhibited decreased PPARα, SREBF1 and AMPK protein expression. Impeded glycerol release into the culture medium was observed after rooibos treatment. None of the concentrations of rooibos hot water soluble solids was cytotoxic, in terms of ATP content. Interestingly, the higher concentration of hot water soluble solids increased ATP concentrations which were associated with increased basal glucose uptake. Decreased leptin secretion was observed after rooibos treatment. Our data show that hot water soluble solids from fermented rooibos inhibit adipogenesis and affect adipocyte metabolism, suggesting its potential in preventing obesity. © 2013 Elsevier GmbH.
Authors & Co-Authors
Sanderson, Micheline
South Africa, Tygerberg
South African Medical Research Council
Mazibuko-Mbeje, Sithandiwe E.
South Africa, Tygerberg
South African Medical Research Council
Joubert, Elizabeth
South Africa, Stellenbosch
Arc Infruitec-nietvoorbij
South Africa, Stellenbosch
Stellenbosch University
De Beer, Dalene
South Africa, Stellenbosch
Arc Infruitec-nietvoorbij
Johnson, Rabia
South Africa, Tygerberg
South African Medical Research Council
Pheiffer, Carmen
South Africa, Tygerberg
South African Medical Research Council
Louw, Johan A.
South Africa, Tygerberg
South African Medical Research Council
Muller, Christo John Frederick
South Africa, Tygerberg
South African Medical Research Council
Statistics
Citations: 65
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/j.phymed.2013.08.011
ISSN:
09447113
e-ISSN:
1618095X
Research Areas
Environmental
Noncommunicable Diseases