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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection and characterization of a novel polyomavirus in wild rodents
Journal of General Virology, Volume 92, No. 4, Year 2011
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Description
To investigate polyomavirus infection in wild rodents, we analysed DNA samples from the spleens of 100 wild rodents from Zambia using a broad-spectrum PCR-based assay. A previously unknown polyomavirus genome was identified in a sample from a multimammate mouse (Mastomys species) and the entire viral genome of 4899 bp was subsequently sequenced. This viral genome contained potential ORFs for the capsid proteins, VP1, VP2 and VP3, and early proteins, small t antigen and large T antigen. Phylogenetic analysis showed that it was a novel member of the family Polyomaviridae, and thus the virus was tentatively named mastomys polyomavirus. After transfection of the viral genome into several mammalian cell lines, transient expression of the VP1 and large T antigen proteins was confirmed by immunoblotting and immunocytochemical analyses. Comparison of large T antigen function in mastomys polyomavirus with that in rhesus monkey polyomavirus SV40 and human polyomavirus JC virus revealed that the large T antigen from mastomys polyomavirus interacted with the tumour suppressor protein pRb, but not with p53. © 2011 SGM.
Authors & Co-Authors
Orba, Yasuko
Japan, Sapporo
Hokkaido University
Kobayashi, Shintaro
Japan, Sapporo
Hokkaido University
Nakamura, Ichiro
Japan, Sapporo
Hokkaido University
Ishii, Akihiro
Zambia, Lusaka
Hokudai Center for Zoonosis Control in Zambia
Hang'Ombe, Bernard Mudenda
Zambia, Lusaka
University of Zambia
Mweene, Aaron Simanyengwe
Zambia, Lusaka
University of Zambia
Thomas, Yuka
Zambia, Lusaka
Hokudai Center for Zoonosis Control in Zambia
Kimura, Takashi
Japan, Sapporo
Hokkaido University
Sawa, Hirofumi
Japan, Sapporo
Hokkaido University
Statistics
Citations: 40
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1099/vir.0.027854-0
ISSN:
00221317
e-ISSN:
14652099
Research Areas
Cancer
Genetics And Genomics
Study Locations
Zambia