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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Tumour-like phenotypes in urothelial cells after exposure to antigens from eggs of Schistosoma haematobium: An oestrogen-DNA adducts mediated pathway?
International Journal for Parasitology, Volume 43, No. 1, Year 2013
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Description
Chronic infection with the blood fluke, Schistosoma haematobium, is associated with squamous cell carcinoma of the bladder. Previously, it has been shown that soluble extracts of mixed sex adult S. haematobium worms (SWAP) are tumourigenic, both in vitro and in vivo. In addition, oestrogen-related molecules in SWAP of S. haematobium down-regulate oestrogen receptors (ERs) alpha and beta in oestrogen responsive cells. Moreover, schistosome oestrogens occur in sera of persons with schistosomiasis haematobia and repress transcription of ERs in urothelial cells. Given that eggs of S. haematobium are the developmental stage directly responsible for urogenital disease during schistosomiasis haematobia, we suspected that soluble antigens from S. haematobium eggs exhibit similar or more potent tumorigenic capacity. Here we investigated the tumorigenic potential of soluble egg antigens (Sh-SEA) of S. haematobium and the endocrine system in favouring parasitism by schistosomes. The findings confirmed that 6.25. μg/ml of Sh-SEA was enough to stimulate cell proliferation, reduce apoptosis and increase oxidative stress of Sh-SEA-exposed urothelial cells. In addition, genotoxic effects of Sh-SEA on these cells were determined by using alkaline single-cell gel electrophoresis (Comet). Furthermore, Liquid Chromatography Diode Array Detection Electron Spray Ionisation Mass Spectrometry indicated the presence of catechol-oestrogens in S. haematobium SEA. A prospective oestrogen-DNA adduct mediated pathway in S. haematobium egg induced bladder cancer is also discussed. © 2012 Australian Society for Parasitology Inc.
Authors & Co-Authors
Botelho, Mónica C.
Portugal, Porto
Universidade do Porto
Portugal, Porto
National Health Institute Doutor Ricardo Jorge Porto
Vale, Nuno
Portugal, Porto
Universidade do Porto
Gouveia, Maria João
Portugal, Porto
Universidade do Porto
Rinaldi, Gabriel
United States, Washington, D.c.
The George Washington University
Uruguay, Montevideo
Universidad de la Republica Facultad de Medicina
Santos, Júlio
Angola, Luanda
Clínica Sagrada Esperança
Santos, Lúcio Lara
Portugal, Porto
Instituto Português de Oncologia do Porto fg
Gomes, Paula
Portugal, Porto
Universidade do Porto
Brindley, Paul J.
United States, Washington, D.c.
The George Washington University
Correia da Costa, José Manuel
Portugal, Porto
Universidade do Porto
Portugal, Porto
National Health Institute Doutor Ricardo Jorge Porto
Statistics
Citations: 47
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1016/j.ijpara.2012.10.023
ISSN:
00207519
e-ISSN:
18790135
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Design
Cohort Study