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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Characterization of polarization states of canine monocyte derived macrophages
PLoS ONE, Volume 18, No. 11 November, Article e0292757, Year 2023
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Description
Macrophages can reversibly polarize into multiple functional subsets depending on their micro-environment. Identification and understanding the functionality of these subsets is relevant for the study of immune-related diseases. However, knowledge about canine macrophage polarization is still in its infancy. In this study, we polarized canine monocytes using GM-CSF/IFN- γ and LPS towards M1 macrophages or M-CSF and IL-4 towards M2 macrophages and compared them to undifferentiated monocytes (M0). Polarized M1 and M2 macrophages were thoroughly characterized for morphology, surface marker features, gene profiles and functional properties. Our results showed that canine M1-polarized macrophages obtained a characteristic large, roundish, or amoeboid shape, while M2-polarized macrophages were smaller and adopted an elongated spindle-like morphology. Phenotypically, all macrophage subsets expressed the pan-macrophage markers CD14 and CD11b. M1-polarized macrophages expressed increased levels of CD40, CD80 CD86 and MHC II, while a significant increase in the expression levels of CD206, CD209, and CD163 was observed in M2-polarized macrophages. RNAseq of the three macrophage subsets showed distinct gene expression profiles, which are closely associated with immune responsiveness, cell differentiation and phagocytosis. However, the complexity of the gene expression patterns makes it difficult to assign clear new polarization markers. Functionally, undifferentiated -monocytes, and M1- and M2- like subsets of canine macrophages can all phagocytose latex beads. M2-polarized macrophages exhibited the strongest phagocytic capacity compared to undifferentiated monocytes- and M1-polarized cells. Taken together, this study showed that canine M1 and M2-like macrophages have distinct features largely in parallel to those of well-studied species, such as human, mouse and pig. These findings enable future use of monocyte derived polarized macrophages particularly in studies of immune related diseases in dogs. Copyright: © 2023 Lyu et al.
Authors & Co-Authors
Lyu, Qingkang
Netherlands, Utrecht
Universiteit Utrecht
United States, Augusta
Augusta University
Veldhuizen, Edwin J.A.
Netherlands, Utrecht
Universiteit Utrecht
Ludwig, Irene Stephanie
Netherlands, Utrecht
Universiteit Utrecht
Rutten, Victor P.M.G.
Netherlands, Utrecht
Universiteit Utrecht
South Africa, Pretoria
University of Pretoria
van Eden, Willem
Netherlands, Utrecht
Universiteit Utrecht
Sijts, Alice J.A.M.
Netherlands, Utrecht
Universiteit Utrecht
Broere, Femke
Netherlands, Utrecht
Universiteit Utrecht
Statistics
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0292757
ISSN:
19326203
Research Areas
Genetics And Genomics