Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Comparative analysis of resistant and susceptible macrophage gene expression response to Leishmania major parasite
BMC Genomics, Volume 14, No. 1, Article 723, Year 2013
Notification
URL copied to clipboard!
Description
Background: Leishmania are obligated intracellular pathogens that replicate almost exclusively in macrophages. The outcome of infection depends largely on parasite pathogenicity and virulence but also on the activation status and genetic background of macrophages. Animal models are essential for a better understanding of pathogenesis of different microbes including Leishmania.Results: Here we compared the transcriptional signatures of resistant (C57BL/6) and susceptible (BALB/c) mouse bone marrow-derived macrophages in response to Leishmania major (L. major) promastigotes infection. Microarray results were first analyzed for significant pathways using the Kyoto Encylopedia of Genes and Genomes (KEGG) database. The analysis revealed that a large set of the shared genes is involved in the immune response and that difference in the expression level of some chemokines and chemokine receptors could partially explain differences in resistance. We next focused on up-regulated genes unique to either BALB/c or C57BL/6 derived macrophages and identified, using KEGG database, signal transduction pathways among the most relevant pathways unique to both susceptible and resistant derived macrophages. Indeed, genes unique to C57BL/6 BMdMs were associated with target of rapamycin (mTOR) signaling pathway while a range of genes unique to BALB/c BMdMs, belong to p53 signaling pathway. We next investigated whether, in a given mice strain derived macrophages, the different up-regulated unique genes could be coordinately regulated. Using GeneMapp Cytoscape, we showed that the induced genes unique to BALB/c or C57BL/6 BMdMs are interconnected. Finally, we examined whether the induced pathways unique to BALB/c derived macrophages interfere with the ones unique to C57BL/6 derived macrophages. Protein-protein interaction analysis using String database highlights the existence of a cross-talk between p53 and mTOR signaling pathways respectively specific to susceptible and resistant BMdMs.Conclusions: Taken together our results suggest that strains specific pathogenesis may be due to a difference in the magnitude of the same pathways and/or to differentially expressed pathways in the two mouse strains derived macrophages. We identify signal transduction pathways among the most relevant pathways modulated by L. major infection, unique to BALB/c and C57BL/6 BMdM and postulate that the interplay between these potentially interconnected pathways could direct the macrophage response toward a given phenotype. © 2013 Rabhi et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Rabhi, Imen
Tunisia, Tunis
Institut Pasteur de Tunis
Rabhi, Sameh
Tunisia, Tunis
Institut Pasteur de Tunis
Ben-Othman, Rym
Tunisia, Tunis
Institut Pasteur de Tunis
Aniba, Mohamed R.
United States, College Park
University of Maryland, College Park
Trentin, Bernadette
France
Skuldtech. Cap Delta - Zac Euromedecine Ii. 1682
Piquemal, D.
France
Skuldtech. Cap Delta - Zac Euromedecine Ii. 1682
Regnault, Béatrice
France, Paris
Institut Pasteur, Paris
Guizani-Tabbane, Lamia
Tunisia, Tunis
Institut Pasteur de Tunis
Attia, Hanène
Unknown Affiliation
Ben Miled, Slimane
Unknown Affiliation
Benkahla, Alia
Unknown Affiliation
Bruno, Roman
Unknown Affiliation
Cazenave, Pierre André
Unknown Affiliation
Checkmeneva, Elena
Unknown Affiliation
Daskalaki, Adriani
Unknown Affiliation
Dellagi, Koussay
Unknown Affiliation
Gabdoulline, Razif
Unknown Affiliation
Ghedira, Kaïs
Unknown Affiliation
Guerfali, Fatma Zahra
Unknown Affiliation
Gustin, Cindy
Unknown Affiliation
Herwig, Ralf
Unknown Affiliation
Hide, Winston A.
Unknown Affiliation
Hofmann, Oliver M.
Unknown Affiliation
Hornischer, Klaus
Unknown Affiliation
Kel, Alexander
Unknown Affiliation
Kiselev, Ilya
Unknown Affiliation
Kolpakov, Fedor A.
Unknown Affiliation
Kondrakhin, Yuriy
Unknown Affiliation
Kutumova, Elena
Unknown Affiliation
Land, Sigrid
Unknown Affiliation
Laouini, Dhafer
Unknown Affiliation
Lemaire, Julien
Unknown Affiliation
Liebich, Ines
Unknown Affiliation
Manchon, Laurent
Unknown Affiliation
Matys, Volker
Unknown Affiliation
Michael, Holger
Unknown Affiliation
Mkannez, Ghada
Unknown Affiliation
Noguier, Florian
Unknown Affiliation
Pierrat, Fabien
Unknown Affiliation
Rasche, Axel
Unknown Affiliation
Renard, Patricia
Unknown Affiliation
Ryabova, Anna
Unknown Affiliation
Jauregui, Ruy
Unknown Affiliation
Schacherer, Frank
Unknown Affiliation
Sghaier, Rabiaa Manel
Unknown Affiliation
Sharipov, Ruslan N.
Unknown Affiliation
Stegmaier, Philip
Unknown Affiliation
Tiffin, Nicki T.
Unknown Affiliation
Tolstykh, Nikita
Unknown Affiliation
Valeev, Tagir
Unknown Affiliation
Voss, Nico
Unknown Affiliation
Wierling, Christoph
Unknown Affiliation
Yevshin, Ivan S.
Unknown Affiliation
Statistics
Citations: 53
Authors: 53
Affiliations: 4
Identifiers
Doi:
10.1186/1471-2164-14-723
e-ISSN:
14712164
Research Areas
Genetics And Genomics