Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure
International Archives of Allergy and Immunology, Volume 142, No. 1, Year 2006
Notification
URL copied to clipboard!
Description
Background: The human IgE response is associated with allergy and with host defence against parasitic worms. A response to Sm22.6, the dominant IgE antigen in adult Schistosoma mansoni worms, correlates with resistance to re-infection after treatment. Sm22.6 is one of a family of EF-hand containing parasite proteins with sequence similarity to dynein light chain (DLC) and with major non-parasite allergens. Here we compare human IgE and IgG responses to other family members, Sm20.8 and Sm21.7, as well as to SmDLC1, relating these to antigen structure and expression in parasite life stages. Methods: Recombinant antigens were used in ELISA to measure antibody isotype responses in 177 cases from an endemic area, before and 7 weeks after treatment. Parasite antigen expression was assessed by RT-PCR and Western blotting. Results: Levels of antibodies to Sm22.6 and Sm20.8 (but not to Sm21.7 or SmDLC1) showed posttreatment increases in all but young children. Many produced IgE to Sm22.6 and Sm20.8 (2 EF-hands), few to Sm21.7 (1 EF-hand) or SmDLC1 (no EF-hands). Sm21.7 was expressed in cercariae, adults and eggs, Sm22.6 and Sm20.8 were concentrated in the adult. Conclusions: These studies suggest that IgE antigens Sm22.6 and Sm20.8 are only released to boost antibodies when adult worms die, whilst Sm21.7 and SmDLC1 are released constantly from eggs dying in host tissue. IgE responses to these allergen-like molecules may be influenced by patterns of exposure and the number of EF-hand motifs. Copyright © 2007 S. Karger AG.
Authors & Co-Authors
Fitzsimmons, Colin Matthew
United Kingdom, Cambridge
University of Cambridge
McBeath, Rowena
United Kingdom, Cambridge
University of Cambridge
Joseph, Sarah
South Africa, Cape Town
University of Cape Town
Jones, Frances M.
United Kingdom, Cambridge
University of Cambridge
Walter, Klaudia
United Kingdom, Cambridge
Cambridge Institute of Public Health
Hoffmann, Karl F.
United Kingdom, Cambridge
University of Cambridge
Kariuki, Henry Curtis
Kenya, Nairobi
Ministry of Health Nairobi
Mwatha, Joseph K.
Kenya, Nairobi
Kenya Medical Research Institute
Kimani, Gachuhi G.
Kenya, Nairobi
Kenya Medical Research Institute
Kabatereine, Narcis B.
Uganda, Kampala
Uganda Ministry of Health
Vennervald, Birgitte Jyding
Denmark, Copenhagen
Dbl -center for Health Research and Development
Ouma, John Henry
Kenya, Nairobi
Ministry of Health Nairobi
Dunne, David William
United Kingdom, Cambridge
University of Cambridge
Statistics
Citations: 71
Authors: 13
Affiliations: 7
Identifiers
Doi:
10.1159/000095997
ISSN:
10182438
Research Areas
Maternal And Child Health