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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Nef-mediated enhancement of virion infectivity and stimulation of viral replication are fundamental properties of primate lentiviruses
Journal of Virology, Volume 81, No. 24, Year 2007
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Description
Nef is a multifunctional accessory protein of primate lentiviruses. Recently, it has been shown that the ability of Nef to downmodulate CD4, CD28, and class I major histocompatibility complex is highly conserved between most or all primate lentiviruses, whereas Nef-mediated downregulation of T-cell receptor-CD3 was lost in the lineage that gave rise to human immunodeficiency virus type 1 (HIV-1). Whether or not other Nef activities are preserved between different groups of primate lentiviruses remained to be determined. Here, we show that nef genes from a large variety of HIVs and simian immunodeficiency viruses (SIVs) enhance virion infectivity and stimulate viral replication in human cells and/or in ex vivo infected human lymphoid tissue (HLT). Notably, nef alleles from unpassaged SIVcpz and SIVsmm enhanced viral infectivity, replication, and cytopathicity in cell culture and in ex vivo infected HLT as efficiently as those from HIV-1 and HIV-2, their human counterparts. Furthermore, nef genes from several highly divergent SIVs that have not been found in humans were also highly active in human cells and/or tissues. Thus, most primate lentiviral Nefs enhance virion infectivity and stimulate viral replication. Moreover, our data show that SIVcpz and SIVsmm Nefs do not require adaptive changes to perform these functions in human cells or tissues and support the idea that nef alleles from other primate lentiviruses would also be capable of promoting efficient virus spread in humans. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Münch, Jan L.
Germany, Ulm
Universität Ulm
Rajan, Devi
Germany, Ulm
Universität Ulm
Schindler, Michael
Germany, Ulm
Universität Ulm
Germany, Hamburg
Heinrich-pette-institut Leibniz-institut Für Experimentelle Virologie
Specht, Anke
Germany, Ulm
Universität Ulm
Rücker, Elke
Germany, Ulm
Universität Ulm
Novembre, Francis J.
United States, Atlanta
Emory University
Nerrienet, Eric
Cameroon, Yaounde
Centre Pasteur du Cameroun
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Müller-Trutwin, Michaela C.
France, Paris
Institut Pasteur, Paris
Peeters, Martine F.
United States, Birmingham
The University of Alabama at Birmingham
Hahn, Beatrice H.
France, Montpellier
Université de Montpellier
Kirchhoff, Frank
Germany, Ulm
Universität Ulm
Statistics
Citations: 11
Authors: 11
Affiliations: 8
Identifiers
Doi:
10.1128/JVI.00904-07
ISSN:
0022538X
Research Areas
Infectious Diseases