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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
engineering
Novel ZnO: Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells
Beilstein Journal of Nanotechnology, Volume 6, No. 1, Year 2015
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Description
The use of photoactive nanoparticles (NPs) such as zinc oxide (ZnO) and its nanocomposites has become a promising anticancer strategy. However, ZnO has a low photocatalytic decomposition rate and the incorporation of metal ions such as silver (Ag) improves their activity. Here different formulations of ZnO:Ag (1, 3, 5, 10, 20 and 30% Ag) were synthesized by a simple co-precipitation method and characterized by powder X-ray diffraction, scanning electron microscopy, Rutherford back scattering and diffuse reflectance spectroscopy for their structure, morphology, composition and optical band gap. The NPs were investigated with regard to their different photocatalytic cytotoxic effects in human malignant melanoma (HT144) and normal (HCEC) cells. The ZnO:Ag nanocomposites killed cancer cells more efficiently than normal cells under daylight exposure. Nanocomposites having higher Ag content (10, 20 and 30%) were more toxic compared to low Ag content (1, 3 and 5%). For HT144, under daylight exposure, the IC50 values were ZnO:Ag (10%): 23.37 μg/mL, ZnO:Ag (20%): 19.95 μg/mL, and ZnO:Ag (30%): 15.78 μg/mL. ZnO:Ag (30%) was toxic to HT144 (IC50: 23.34 μg/mL) in dark as well. The three nanocomposites were further analyzed with regard to their ability to generate reactive oxygen species (ROS) and induce lipid peroxidation. The particles led to an increase in levels of ROS at cytotoxic concentrations, but only HT144 showed strongly induced MDA level. Finally, NPs were investigated for the ROS species they generated in vitro. A highly significant increase of 1O2 in the samples exposed to daylight was observed. Hydroxyl radical species, HO•, were also generated to a lesser extent. Thus, the incorporation of Ag into ZnO NPs significantly improves their photo-oxidation capabilities. ZnO:Ag nanocomposites could provide a new therapeutic option to selectively target cancer cells. © 2015 Arooj et al; licensee Beilstein-Institut.
Authors & Co-Authors
Nazir, Samina
Pakistan, Islamabad
Quaid-i-azam University
Nadhman, Akhtar
Pakistan, Islamabad
Quaid-i-azam University
Ahmad, Nafees H.T.H.
Pakistan, Islamabad
Institute of Biomedical and Genetic Engineering
Muhammad, Bakhtiar
Unknown Affiliation
Ahmad, I.
Pakistan, Islamabad
Quaid-i-azam University
Abbasi, Rashda
Pakistan, Islamabad
Institute of Biomedical and Genetic Engineering
Statistics
Citations: 53
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.3762/bjnano.6.59
ISSN:
21904286
Research Areas
Cancer
Environmental