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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
veterinary
Effects of high-mobility group a protein application on canine adipose-derived mesenchymal stem cells in vitro
Veterinary Medicine International, Article 752083, Year 2012
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Description
Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated. Therefore, extracellular HMGA1 and HMGA2 application alone (10-200ng/mL) and in combination with each other (100, 200ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation. Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100ng/mL) was analyzed by RT-qPCR. HMGA1 significantly reduced cASCs proliferation in concentrations of 10-200ng/mL culture medium. A combination of HMGA1 and HMGA2 protein (100 and 200ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone. RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs. Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities. © 2012 A. A. Ismail et al.
Authors & Co-Authors
Abdelbaset-Ismail, Ahmed Attia
Germany, Hannover
Tierärztliche Hochschule Hannover
Egypt, Zagazig
Faculty of Veterinary Medicine
Wagner, Siegfried
Germany, Hannover
Tierärztliche Hochschule Hannover
Germany, Bremen
Universität Bremen
Murua-Escobar, Hugo
Germany, Hannover
Tierärztliche Hochschule Hannover
Willenbrock, Saskia
Germany, Hannover
Tierärztliche Hochschule Hannover
Germany, Bremen
Universität Bremen
Sterenczak, Katharina Anna
Germany, Hannover
Tierärztliche Hochschule Hannover
Germany, Bremen
Universität Bremen
Samy, Mohamed Tayiser
Egypt, Zagazig
Faculty of Veterinary Medicine
Abdel-Aal, A. M.
Egypt, Zagazig
Faculty of Veterinary Medicine
Nolte, Ingo J.A.
Germany, Hannover
Tierärztliche Hochschule Hannover
Wefstaedt, Patrick
Germany, Hannover
Tierärztliche Hochschule Hannover
Statistics
Citations: 9
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1155/2012/752083
e-ISSN:
20420048
Research Areas
Cancer