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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The phytosulfokine (PSK) receptor is capable of guanylate cyclase activity and enabling cyclic GMP-dependent signaling in plants
Journal of Biological Chemistry, Volume 286, No. 25, Year 2011
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Description
Phytosulfokines (PSKs) are sulfated pentapeptides that stimulate plant growth and differentiation mediated by the PSK receptor (PSKR1), which is a leucine-rich repeat receptor-like kinase. We identified a putative guanylate cyclase (GC) catalytic center in PSKR1 that is embedded within the kinase domain and hypothesized that the GC works in conjunction with the kinase in downstream PSK signaling. We expressed the recombinant complete kinase (cytoplasmic) domain of AtPSKR1 and show that it has serine/threonine kinase activity using the Ser/Thr peptide 1 as a substrate with an approximate Km of 7.5 μM and Vmax of 1800 nmol min-1 mg-1 of protein. This same recombinant protein also has GC activity in vitro that is dependent on the presence of either Mg2+ or Mn2+. Overexpression of the full-length AtPSKR1 receptor in Arabidopsis leaf protoplasts raised the endogenous basal cGMP levels over 20-fold, indicating that the receptor has GC activity in vivo. In addition, PSK-α itself, but not the non-sulfated backbone, induces rapid increases in cGMP levels in protoplasts. Together these results indicate that the PSKR1 contains dual GC and kinase catalytic activities that operate in vivo and that this receptor constitutes a novel class of enzymes with overlapping catalytic domains. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3121402/bin/supp_286_25_22580__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3121402/bin/supp_M110.168823_SuppFig1_Kwezi.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3121402/bin/supp_M110.168823_SuppFig2_Kwezi.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3121402/bin/supp_M110.168823_Supplementary_table_1_Kwezi.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3121402/bin/supp_M110.168823_Supplementary_methods_Kwezi.pdf
Authors & Co-Authors
Kwezi, Lusisizwe
Australia, Melbourne
Monash Institute of Pharmaceutical Sciences
South Africa, Bellville
University of the Western Cape
Ruzvidzo, Oziniel
South Africa, Bellville
University of the Western Cape
South Africa, Potchefstroom
North-west University
Wheeler, Janet I.
Australia, Melbourne
Monash Institute of Pharmaceutical Sciences
Govender, Kershini
South Africa, Bellville
University of the Western Cape
Iacuone, Sylvana
Australia, Melbourne
Monash Institute of Pharmaceutical Sciences
Thompson, Philip E.
Australia, Melbourne
Monash Institute of Pharmaceutical Sciences
Gehring, Chris A.
South Africa, Bellville
University of the Western Cape
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Irving, Helen R.
Australia, Melbourne
Monash Institute of Pharmaceutical Sciences
Statistics
Citations: 124
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1074/jbc.M110.168823
ISSN:
00219258
e-ISSN:
1083351X