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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The effect of intracavernous injection of adipose tissue-derived stem cells on hyperlipidemia-associated erectile dysfunction in a rat model
Journal of Sexual Medicine, Volume 7, No. 4 PART 1, Year 2010
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Description
Introduction.: Hyperlipidemia has been associated with erectile dysfunction (ED) via damage to the cavernous endothelium and nerves. Adipose tissue-derived stem cells (ADSC) have been shown to differentiate into endothelial cells and secrete vasculotrophic and neurotrophic factors. Aim.: To assess whether ADSC have therapeutic effects on hyperlipidemia-associated ED. Methods.: Twenty-eight male rats were induced to develop hyperlipidemia with a high-fat diet (hyperlipidemic rats, HR). Ten additional male rats were fed a normal diet to serve as controls (normal rats, NR). Five months later, all rats were subjected to ADSC isolation from paragonadal fat. The cells were cultured for 1 week, labeled with 5-ethynyl-2′-deoxyuridine (EdU), and then injected autologously into the corpus cavernosum of 18 HR. The remaining 10 HR rats were injected with phosphate buffered saline (PBS). At 2 and 14 days post-transplantation, four rats in the HR + ADSC group were sacrificed for tracking of the transplanted cells. At 28 days post-transplantation, all remaining rats were analyzed for serum biochemistry, erectile function, and penile histology. Main Outcome Measures.: Erectile function was assessed by intracavernous pressure (ICP) measurement during electrostimulation of the cavernous nerve. Cavernous nerves, endothelium, and smooth muscle were assessed by immunohistochemistry. Results.: Serum total cholesterol and low-density lipoprotein levels were significantly higher in HR than in NR. High-density lipoprotein level was significantly lower in HR than in NR. Mean ICP/mean arterial pressure ratio was significantly lower in HR + PBS than in NR + PBS or HR + ADSC. Neuronal nitric oxide synthase (nNOS)-positive nerve fibers and endothelial cells were fewer in HR + PBS than in HR + ADSC. Smooth muscle content was significantly higher in both HR groups than in NR. Conclusions.: Hyperlipidemia is associated with abnormalities in both the nerves and endothelium. Treatment with ADSC ameliorates these adverse effects and holds promise as a potential new therapy for ED. Huang Y-C, Ning H, Shindel AW, Fandel TM, Lin G, Harraz AM, Lue TF, and Lin C-S. The effect of intracavernous injection of adipose tissue-derived stem cells on hyperlipidemia-associated erectile dysfunction in a rat model. © 2010 International Society for Sexual Medicine.
Authors & Co-Authors
Huang, Yunching
United States, San Francisco
University of California, San Francisco
Taiwan, Taipei
Chang Gung Memorial Hospital
Taiwan, Taoyuan
Chang Gung University
Shindel, Alan W.
United States, San Francisco
University of California, San Francisco
Fandel, Thomas M.
United States, San Francisco
University of California, San Francisco
Lin, Guiting
United States, San Francisco
University of California, San Francisco
Harraz, Ahmed M.
United States, San Francisco
University of California, San Francisco
Lue, Thomas F.
United States, San Francisco
University of California, San Francisco
Lin, Ching Schwun
United States, San Francisco
University of California, San Francisco
Statistics
Citations: 99
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1111/j.1743-6109.2009.01697.x
ISSN:
17436095
Research Areas
Cancer
Health System And Policy
Sexual And Reproductive Health
Participants Gender
Male