Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Differentiation of primary cytomegalovirus infection from reactivation using the urea denaturation test for measuring antibody avidity
Journal of Medical Virology, Volume 33, No. 1, Year 1991
Notification
URL copied to clipboard!
Description
Cytomegalovirus (CMV) is probably the most common agent of prenatal infection of the new‐born, and one of 20 congenitally infected newborns shows serious symptoms. It was therefore considered important to be able to differentiate primary CMV from reactivation in pregnant females. A urea denaturation test was used to distinguish primary from secondary rubella infection in which the urea is included in the wash step of the standard IgG ELISA. This resulted in the removal of low‐avidity antibodies, which are the antibodies produced early in infection. A group of CMV IgM‐negative and ‐positive sera were tested, and all but one showed moderate to high avidity, with an avidity index reading of more than 30%. Among a group of babies 3–12 months of age, who were CMV IgM positive, 55% (16 of 29) showed low‐avidity CMV antibodies. A small group of renal transplant patients and patients with clinically and laboratory‐confirmed CMV gave more or less predicted avidity index results. It appears that, with the method used at this laboratory, the urea denaturation test can be applied to CMV to determine primary infection or reactivation in the majority of cases. Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company
Authors & Co-Authors
Blackburn, Nigel K.
South Africa, Johannesburg
University of the Witwatersrand
Besselaar, Terry G.
South Africa, Johannesburg
University of the Witwatersrand
Schoub, Barry David
South Africa, Johannesburg
University of the Witwatersrand
O'Connell, K. F.
South Africa, Johannesburg
University of the Witwatersrand
Statistics
Citations: 124
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1002/jmv.1890330103
ISSN:
01466615
e-ISSN:
10969071
Research Areas
Maternal And Child Health
Participants Gender
Female