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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Prevalence of diarrheagenic Escherichia coli virulence genes in the feces of slaughtered cattle, chickens, and pigs in Burkina Faso
MicrobiologyOpen, Volume 1, No. 3, Year 2012
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Description
We investigated the prevalence of the virulence genes specific for five major pathogroups of diarrheagenic Escherichia coli (DEC) in primary cultures from feces of animals slaughtered for human consumption in Burkina Faso. For the study, 704 feces samples were collected from cattle ( = 304), chickens ( = 350), and pigs ( = 50) during carcass processing. The presence of the virulence-associated genes in the mixed bacterial cultures was assessed using 16-plex polymerase chain reaction (PCR). Virulence genes indicating presence of DEC were detected in 48% of the cattle, 48% of the chicken, and 68% of the pig feces samples. Virulence genes specific for different DECs were detected in the following percentages of the cattle, chicken, and pig feces samples: Shiga toxin-producing E. coli (STEC) in 37%, 6%, and 30%; enteropathogenic E. coli (EPEC) in 8%, 37%, and 32%; enterotoxigenic E. coli (ETEC) in 4%, 5%, and 18%; and enteroaggregative E. coli (EAEC) in 7%, 6%, and 32%. Enteroinvasive E. coli (EIEC) virulence genes were detected in 1% of chicken feces samples only. The study was the first of its kind in Burkina Faso and revealed the common occurrence of the diarrheal virulence genes in feces of food animals. This indicates that food animals are reservoirs of DEC that may contaminate meat because of the defective slaughter and storage conditions and pose a health risk to the consumers in Burkina Faso. © 2012 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.
Authors & Co-Authors
Kagambèga, Assèta
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Burkina Faso, Ouagadougou
University of Ouagadougou
Martikainen, Outi
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Siitonen, Anja
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Traoré, Alfred Sababénédjo
Burkina Faso, Ouagadougou
University of Ouagadougou
Barro, Nicolas
Burkina Faso, Ouagadougou
University of Ouagadougou
Haukka, Kaisa E.
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Finland, Helsinki
Helsingin Yliopisto
Statistics
Citations: 59
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1002/mbo3.30
e-ISSN:
20458827
Research Areas
Food Security
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Burkina Faso