Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Hierarchy among viral RNA (vRNA) segments in their role in vRNA incorporation into influenza A virions
Journal of Virology, Volume 80, No. 5, Year 2006
Notification
URL copied to clipboard!
Description
The genome of influenza A viruses comprises eight negative-strand RNA segments. Although all eight segments must be present in cells for efficient viral replication, the mechanism(s) by which these viral RNA (vRNA) segments are incorporated into virions is not fully understood. We recently found that sequences at both ends of the coding regions of the HA, NA, and NS vRNA segments of A/WSN/33 play important roles in the incorporation of these vRNAs into virions. In order to similarly identify the regions of the PB2, PB1, and PA vRNAs of this strain that are critical for their incorporation, we generated a series of mutant vRNAs that possessed the green fluorescent protein gene flanked by portions of the coding and noncoding regions of the respective segments. For all three polymerase segments, deletions at the ends of their coding regions decreased their virion incorporation efficiencies. More importantly, these regions not only affected the incorporation of the segment in which they reside, but were also important for the incorporation of other segments. This effect was most prominent with the PB2 vRNA. These findings suggest a hierarchy among vRNA segments for virion incorporation and may imply intersegment association of vRNAs during virus assembly. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Takada, Ayato
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Noda, Takeshi
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Iwatsuki-Horimoto, Kiyoko
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Watanabe, Shinji
United States, Madison
University of Wisconsin-madison
Horimoto, Taisuke
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Kida, Hiroshi
Japan, Sapporo
Hokkaido University
Kawaoka, Yoshihiro K.
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
United States, Madison
University of Wisconsin-madison
Statistics
Citations: 153
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1128/JVI.80.5.2318-2325.2006
ISSN:
0022538X
Research Areas
Genetics And Genomics