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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Isolation and sequence analysis of a cDNA encoding the c subunit of a vacuolar-type H
+
-ATPase from the CAM plant Kalanchoë daigremontiana
Plant Molecular Biology, Volume 31, No. 2, Year 1996
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Description
We report the sequence of a cDNA clone encoding the c ('16 kDa') subunit of a vacuolar-type H+-ATPase (V-ATPase) from Kalanchoë daigremontiana, a plant in which the cell vacuole plays a pivotal role in crassulacean acid metabolism. The clone, pKVA211, was isolated from a K. daigremontiana leaf cDNA library constructed in λZAP II using a homologous PCR-generated cDNA probe for the V-ATPase c subunit. The KVA211 cDNA was 839 nucleotides long and included a 20 bp poly(A)+ tail together with a complete 495 bp coding region for a polypeptide with a predicted molecular mass of 16 659 Da. The deduced amino acid sequence was highly conserved across the wide range of eukaryotes (vertebrates, invertebrates, fungi, plants and protozoa) in which this gene has now been identified. Sequence comparison of several PCR products and genomic Southern analysis indicated that the V-ATPase c subunit in K. daigremontiana is encoded by a small multi-gene family. Steady-state levels of the KVA211 mRNA were much higher in leaves than in roots or flowers, and expression of this transcript in leaves was shown to be strongly light-dependent. © 1996 Kluwer Academic Publishers.
Authors & Co-Authors
Bartholomew, Dolores M.
United Kingdom, Oxford
University of Oxford
Rees, David Jasper Gilbert
United Kingdom, Oxford
Sir William Dunn School of Pathology
South Africa, Bellville
University of the Western Cape
Rambaut, Andrew
United Kingdom, Oxford
University of Oxford
Smith, James Andrew Charles
United Kingdom, Oxford
University of Oxford
Statistics
Citations: 10
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1007/BF00021806
ISSN:
01674412
Research Areas
Genetics And Genomics