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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Serotyping HIV Type 1 by Antibody Binding to the V3 Loop: Relationship to Viral Genotype
AIDS Research and Human Retroviruses, Volume 10, No. 11, Year 1994
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Description
We have investigated whether peptides representing the HIV-1 principal neutralization domain (V3) can be used as antigens in antibody-binding assays to predict the genotypes of the subjects’ virus. Serum samples collected from HIV-1-infected subjects from the four WHO-sponsored vaccine evaluation sites (Uganda, Rwanda, Thailand, and Brazil) were characterized by antibody binding to a panel of synthetic V3 peptides that were derived from the consensus sequences of the V3 region of the HIV-1 subgroups according to the env phylogenetic analysis (A-E). An indirect V3 peptide-binding assay was used for primary screening, and a V3 peptide antigen-limiting ELISA was then used as a secondary assay to discriminate cross-reactivity if the screening assay was equivocal. In general, V3 peptide serology could predict HIV-1 genotypes. In sera for which the genotype of the virus was known, peptide assays could predict the correct genotype in approximately 90% of cases for genotypes A, B, C, and E; Ugandan sera of genotype D were more broadly reactive. There was considerable serological cross-reactivity between some HIV-1 genotypes, in particular between A and C, and, to a lesser extent, B and D subtypes. Owing to polymorphism at the crown of the V3 loop, an additional B peptide (B’) was required to type Brazilian B genotype sera. These simple assays may help facilitate the determination and distribution of HIV-1 genotypes circulating in populations. © 1994, Mary Ann Liebert, Inc. All rights reserved.
Authors & Co-Authors
Cheingsong-Popov, Rachanee
United Kingdom, London
Imperial College London
Callow, David
United Kingdom, London
Imperial College London
Kaleebu, Pontiano P.
United Kingdom, London
Imperial College London
Beddows, Simon A.
United Kingdom, London
Imperial College London
Weber, Jonathan N.
United Kingdom, London
Imperial College London
Statistics
Citations: 104
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1089/aid.1994.10.1379
ISSN:
08892229
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Rwanda
Uganda