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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Isolation and partial characterization of the entire human proα I(II) collagen gene
Nucleic Acids Research, Volume 13, No. 7, Year 1985
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Description
Using a cDNA probe specific for the bovine Type II procollagen, a series of overlapping genomic clones containing 45 kb of contiguous human DNA have been isolated. Sequencing of a 54 bp exon, number 29, provided direct evidence that the recombinant clones bear human Type II collagen sequences. Localization of the 5′ and 3′ ends of the gene indicated that the human Type II collagen gene is 30 kb in size. This value is significantly higher than that of the homologous avian gene. The segregation of a polymorphic restriction site in informative families conclusively demonstrated that the Type II gene is found in a single copy in the human haploid genome. Finally, sequencing of a triple helical domain exon has confirmed that a rearrangement leading to the fusion of two exons occurred in the proαl(I) gene, following the divergence of the fibrillar collagens. © 1985 IRL Press Limited.
Authors & Co-Authors
Sangiorgi, Frank O.
United States, Newark
Rutgers Biomedical and Health Sciences
Benson-Chanda, Virginia
United States, Newark
Rutgers Biomedical and Health Sciences
de Wet, Wouter J.
United States, Newark
Rutgers Biomedical and Health Sciences
South Africa, Potchefstroom
North-west University
Sobel, Mark E.
United States, Newark
Rutgers Biomedical and Health Sciences
United States, Bethesda
National Institutes of Health Nih
Tsipouras, Petros
United States, Newark
Rutgers Biomedical and Health Sciences
Ramirez, Francesco
United States, Newark
Rutgers Biomedical and Health Sciences
Statistics
Citations: 63
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1093/nar/13.7.2207
ISSN:
03051048
Research Areas
Genetics And Genomics