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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Resequencing microarray method for molecular diagnosis of human arboviral diseases
Journal of Clinical Virology, Volume 56, No. 3, Year 2013
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Description
Background: Resequencing DNA microarray (RMA) technology uses probes designed to identify a panel of viral sequences. It can be used for detecting emerging viruses by revealing the nucleotide polymorphisms within the target of interest. Objectives/study design: As a new tool for molecular diagnosis of arbovirus infection, high density PathogenID v2.0 RMA (PID2-RMA) was assessed for the detection and genetic analysis of dengue, West Nile, and Chikungunya viruses in spiked blood samples or sera from individuals infected with dengue virus. Viral RNAs extracted from biological samples were retrotranscribed into cDNA and amplified using the Phi 29 polymerase-based method. This amplified cDNA was used for hybridization on PID2-RMA. Results: A good specificity of RMA-based detection was demonstrated using a panel of arboviruses including Dengue, West Nile and Chikungunya viruses. This technology was also efficient for the detection and genetic analysis of the different serotypes of dengue virus in sera of infected patients. Furthermore, the mixing of dengue, West Nile and Chikungunya prototype viruses within a single sample of human blood did not interfere with the sensitivity of PID2-RMA. Conclusions: Our data show that high density PID2-RMA was suitable for the identification of medically important arboviruses. It appears to be particularly adapted to the genetic analysis of dengue, West Nile, and Chikungunya viruses in urgent clinical situations where the rapid identification and characterization of the pathogen is essential. © 2012 Elsevier B.V.
Authors & Co-Authors
Berthet, Nicolas
France, Paris
Institut Pasteur, Paris
France, Paris
Cnrs Centre National de la Recherche Scientifique
Paulous, Sylvie
France, Paris
Institut Pasteur, Paris
Coffey, Lark L.A.
France, Paris
Institut Pasteur, Paris
Frenkiel, Marie Pascale
France, Paris
Institut Pasteur, Paris
Moltini, Isabelle
France, Paris
Institut Pasteur, Paris
Tran, Coralie
France, Paris
Institut Pasteur, Paris
Mathéus, Séverine
French Guiana, Cayenne
Institut Pasteur de la Guyane
Ottone, Catherine
France, Paris
Institut Pasteur, Paris
Ungeheuer, Marie Noëlle
France, Paris
Institut Pasteur, Paris
Caro, Valérie
France, Paris
Institut Pasteur, Paris
Dussart, Philippe
French Guiana, Cayenne
Institut Pasteur de la Guyane
Gessain, Antoine
France, Paris
Institut Pasteur, Paris
France, Paris
Cnrs Centre National de la Recherche Scientifique
Després, Philippe
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 17
Authors: 13
Affiliations: 3
Identifiers
Doi:
10.1016/j.jcv.2012.10.022
ISSN:
13866532
Research Areas
Genetics And Genomics
Infectious Diseases