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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of Neospora caninum in dog organs using real time PCR systems
Veterinary Parasitology, Volume 155, No. 1-2, Year 2008
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Description
Neospora caninum is a parasite responsible for paresis in dogs. The dog can harbour enkysted parasites in several organs. The detection of N. caninum was performed using 3 different real time PCR systems all amplifying the NC5 DNA region. One system was based on Sybr®green, one on Plexor™ technology and the last on Taqman® probe. Comparison of the three methods indicated that the detection limit was 1 equivalent genome on pure DNA but that this detection limit increased in the presence of foreign DNA using the Sybrgreen and Plexor systems. Therefore, the Taqman system was chosen to detect N. caninum in liver and spleen of naturally infected dogs. The overall prevalence was 32.2%. Comparison between PCR results and serological results using IFAT showed that among the 28 PCR positive dogs only 9 were seropositive and that 8 seropositive dogs were PCR negative. Therefore serology can underestimate the real carriage in dogs. However, PCR methods must be improved in terms of sensitivity and inhibition problems. © 2008 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Ghalmi, Farida
Belgium, Liege
Université de Liège
Algeria, Algiers
École Nationale Supérieure Vétérinaire D'alger
China, Bernard
Belgium, Liege
Université de Liège
Belgium, Brussels
Scientific Institute of Public Health, Brussels
Kaidi, Rachid
Algeria, Blida
Université Blida 1
Daube, Georges
Belgium, Liege
Université de Liège
Losson, Bertrand
Belgium, Liege
Université de Liège
Statistics
Citations: 33
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.vetpar.2008.04.007
ISSN:
03044017
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study