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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The identity of conformational states of reconstituted and native histone octamers
European Journal of Biochemistry, Volume 137, No. 1-2, Year 1983
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Description
Histone octamers were reconstituted from the following preparations: (a) natural histone H3‐H4 tetramer and histone H2A‐H2B dimer, either selectively extracted from chromatin with solutions of sodium chloride or prepared by dissociation of the natural octamer; (b) acid‐denatured core histones, either an unfractionated mixture or individually purified proteins. Complexes assembled from these histones elute from exclusion chromatography columns with octamer size as verified by cross‐linking with dimethylsuberimidate. The reconstituted octamers all crystallize in the same form of helical tubes as the natural octamer. Copyright © 1983, Wiley Blackwell. All rights reserved
Authors & Co-Authors
Greyling, H. Johann
South Africa, Cape Town
University of Cape Town
Schwager, Sylva L.U.
South Africa, Cape Town
University of Cape Town
Sewell, Trevor Trevor
South Africa, Cape Town
University of Cape Town
von Holt, Claus
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 17
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1111/j.1432-1033.1983.tb07818.x
ISSN:
00142956
e-ISSN:
14321033