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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Genotyping Trichomonas vaginalis
International Journal for Parasitology, Volume 36, No. 7, Year 2006
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Description
A genotyping method has been developed to distinguish each Trichomonas vaginalis isolate and has provided the first genome mapping studies of this protist with an estimated 180 Mb genome. The technique was developed using high molecular weight DNA prepared from five laboratory isolates from Australia and USA and 20 clinical isolates from South Africa. Inhibition of the notorious T. vaginalis endogenous nucleases by addition of potent inhibitors was essential to the success of this study. Chromosomal DNA larger than 2.2 Mb was macrorestricted to a minimum segment size of ∼50 kb, separated by pulsed field gel electrophoresis and hybridised with a variety of gene probes. Each isolate generated a unique pattern that was distinguished by each of the probes. Four single copy gene probes (fd, hmp35, ibp39 and pfoD) were identified but probes which identified several bands (pfoB and α-scs) per isolate were most informative for genotyping. The pyruvate:ferredoxin oxidoreductase B gene probe identified two to seven copies of pfoB (or its closely related homologue pfoA) per genome in different isolates and is an obvious candidate probe to identify epidemiological linkage between infections by this genotyping method. Cleavage of the genomes into smaller fragments failed to distinguish isolates from diverse locations indicating the proximal regions of genes are conserved. © 2006 Australian Society for Parasitology Inc.
Authors & Co-Authors
Upcroft, Jacqueline A.
Australia, Brisbane
The University of Queensland
Delgadillo-Correa, Maria G.
United States, Los Angeles
David Geffen School of Medicine at Ucla
Dunne, Rebecca L.
Australia, Brisbane
The University of Queensland
Sturm, Adriaan Willem
South Africa, Durban
Faculty of Medicine
Johnson, Patricia J.
United States, Los Angeles
David Geffen School of Medicine at Ucla
Upcroft, Peter
Australia, Brisbane
The University of Queensland
Statistics
Citations: 35
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.ijpara.2006.02.018
ISSN:
00207519
Research Areas
Genetics And Genomics
Study Locations
South Africa