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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Genetic diversity of Ehrlichia ruminantium in Amblyomma variegatum ticks and small ruminants in The Gambia determined by restriction fragment profile analysis
Veterinary Microbiology, Volume 126, No. 1-3, Year 2008
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Description
Understanding genetic diversity of Ehrlichia ruminantium in host and vector populations is an important prerequisite to controlling heartwater by vaccination in traditional livestock systems in sub-Saharan Africa. We carried out a study in two phases: (i) evaluating the usefulness of the PCR-RFLP assay based on the map1 coding sequence of E. ruminantium as a discriminatory tool to characterise genetic diversity, (ii) applying the technique to field samples from Amblyomma variegatum ticks and small ruminants to characterise genotypic diversity of the organism in three main agroecological zones of The Gambia, Sudano-Guinean (SG), Western Sudano-Sahelian (WSS) and Eastern Sudano-Sahelian (ESS). Restriction fragment length polymorphisms were observed among different strains of E. ruminantium supporting the usefulness of the PCR-RFLP technique for studying genetic diversity of the organism. Restriction enzyme map1 profile analysis indicated the presence in The Gambia of multiple genotypes (at least 11) of E. ruminantium with sites in the WSS and SG zones showing comparatively high number of diverse genotypes. Profiles similar to the Kerr Seringe genotype (DQ333230) showed the highest distribution frequency, being present at sites in all three agroecological zones, thereby making the strain a suitable candidate for further characterisation in cross-protection studies. An additional three genotypes showed relatively high distribution frequency and were present in all three zones making them equally important for isolation and subsequent characterisation. The study demonstrated the occurrence of mixed infections with E. ruminantium genotypes in ruminants and ticks. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Faburay, Bonto
Gambia, Banjul
International Trypanotolerance Centre Gambia
Netherlands, Utrecht
Universiteit Utrecht
Jongejan, Frans
Netherlands, Utrecht
Universiteit Utrecht
South Africa, Pretoria
University of Pretoria
Taoufik, Amar
Netherlands, Utrecht
Universiteit Utrecht
Ceesay, Ansumana
Gambia, Banjul
International Trypanotolerance Centre Gambia
Geysen, Dirk M.
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Statistics
Citations: 5
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.vetmic.2007.06.010
ISSN:
03781135
Research Areas
Genetics And Genomics
Study Locations
Gambia