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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations (Mali and Kenya)
BMC Genomics, Volume 7, Article 226, Year 2006
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Description
Background: Salivary proteins from sandflies are potential targets for exploitation as vaccines to control Leishmania infection; in this work we tested the hypothesis that salivary proteins from geographically distant Phlebotomus duboscqi sandfly populations are highly divergent due to the pressure exerted by the host immune response. Salivary gland cDNA libraries were prepared from wild-caught P. duboscqi from Mali and recently colonised flies of the same species from Kenya. Results: Transcriptome and proteome analysis resulted in the identification of the most abundant salivary gland-secreted proteins. Orthologues of these salivary proteins were identified by phylogenetic tree analysis. Moreover, comparative analysis between the orthologues of these two different populations resulted in a high level of protein identity, including the predicted MHC class II T-cell epitopes from all these salivary proteins. Conclusion: These data refute the hypothesis that salivary proteins from geographically distinct populations of the same Phlebotomus sandfly species are highly divergent. They also suggest the potential for using the same species-specific components in a potential vector saliva-based vaccine. © 2006 Kato et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Kato, Hirotomo
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Japan, Yamaguchi
Yamaguchi University
Anderson, Jennifer M.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Kamhawi, Shaden A.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Oliveira, F.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Brazil, Salvador
Universidade Federal da Bahia
Lawyer, Phillip G.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Pham, Van My
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Sangare, Constance Souko
Mali, Bamako
University of Bamako
Samake, Sibiry
Mali, Bamako
University of Bamako
Sissoko, Ibrahim Moussa
Mali, Bamako
University of Bamako
Garfield, Mark K.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Sigutova, Lucie
Czech Republic, Prague
Charles University
Volf, Petr
Czech Republic, Prague
Charles University
Doumbia, Seydou O.
Mali, Bamako
University of Bamako
Valenzuela, Jesús G.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 82
Authors: 14
Affiliations: 5
Identifiers
Doi:
10.1186/1471-2164-7-226
ISSN:
14712164
e-ISSN:
14712164
Research Areas
Genetics And Genomics
Study Locations
Kenya
Mali