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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
The gene expression landscape of pine seedling tissues
Plant Journal, Volume 91, No. 6, Year 2017
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Description
Conifers dominate vast regions of the Northern hemisphere. They are the main source of raw materials for timber industry as well as a wide range of biomaterials. Despite their inherent difficulties as experimental models for classical plant biology research, the technological advances in genomics research are enabling fundamental studies on these plants. The use of laser capture microdissection followed by transcriptomic analysis is a powerful tool for unravelling the molecular and functional organization of conifer tissues and specialized cells. In the present work, 14 different tissues from 1-month-old maritime pine (Pinus pinaster) seedlings have been isolated and their transcriptomes analysed. The results increased the sequence information and number of full-length transcripts from a previous reference transcriptome and added 39 841 new transcripts. In total, 2376 transcripts were ubiquitously expressed in all of the examined tissues. These transcripts could be considered the core ‘housekeeping genes’ in pine. The genes have been clustered in function to their expression profiles. This analysis reduced the number of profiles to 38, most of these defined by their expression in a unique tissue that is much higher than in the other tissues. The expression and localization data are accessible at ConGenIE.org (http://v22.popgenie.org/microdisection/). This study presents an overview of the gene expression distribution in different pine tissues, specifically highlighting the relationships between tissue gene expression and function. This transcriptome atlas is a valuable resource for functional genomics research in conifers. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd
Authors & Co-Authors
Li, Zhen
Belgium, Ghent
Universiteit Gent
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Ávila, Concepción
Spain, Malaga
Universidad de Málaga
Sterck, Lieven
Belgium, Ghent
Universiteit Gent
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Van de Peer, Y.
Belgium, Ghent
Universiteit Gent
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Cánovas, Francisco M.
Spain, Malaga
Universidad de Málaga
Statistics
Citations: 25
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/tpj.13617
ISSN:
09607412
Research Areas
Cancer
Genetics And Genomics