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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle
Molecular Reproduction and Development, Volume 78, No. 4, Year 2011
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Description
Melatonin is a multifunctional molecule that mediates several circadian and seasonal reproductive processes. The exact role of melatonin in modulating reproduction, however, is not fully understood-especially its effects on the ovarian follicles and oocytes. This study was conducted to investigate the expressions of the ASMT and melatonin-receptor MTNR1A and MTNR1B genes in bovine oocytes and their cumulus cells, as well as the effects of melatonin on oocyte nuclear and cytoplasmic maturation in vitro. Cumulus-oocyte complexes (COCs) from abattoir ovaries were cultured in TCM-199 supplemented with melatonin at concentrations of 0, 10, 50, and 100ng/ml. The expression of ASMT, MTNR1A, and MTNR1B genes was evaluated by RT-PCR. Moreover, the effects of melatonin on cumulus cell expansion, nuclear maturation, mitochondrial characteristics and COCs steroidogenesis were investigated. Furthermore, the level of reactive oxygen species (ROS) was evaluated in denuded oocytes. Our study revealed that ASMT and MTNR1A genes were expressed in COCs, while the MTNR1B gene was expressed only in oocytes. Additionally, melatonin supplementation at 10 and 50ng/ml to in vitro maturation medium significantly enhanced oocyte nuclear maturation, cumulus cell expansion and altered the mitochondrial distribution patterns, but had no effects on oocyte mitochondrial activity and COCs steroidogenesis. Melatonin-treated oocytes had a significantly lower level of ROS than controls. The presence of melatonin receptors in COCs and its promoting effects on oocyte nuclear and cytoplasmic events, indicate the potentially important roles of this hormone in regulating bovine oocyte maturation. Moreover, the presence of ASMT transcript in COCs suggests the possible involvement of these cells in melatonin biosynthesis. © 2011 Wiley-Liss, Inc.
Authors & Co-Authors
El-Raey, Mohamed
Japan, Tsukuba
Institute of Livestock and Grassland Science, Naro
Egypt, Benha
Faculty of Veterinary Medicine Benha University
Geshi, Masaya
Japan, Tsukuba
Institute of Livestock and Grassland Science, Naro
Somfai, Tamás
Japan, Tsukuba
Institute of Livestock and Grassland Science, Naro
Kaneda, Masahiro
Japan, Tsukuba
Institute of Livestock and Grassland Science, Naro
Hirako, Makoto
Japan, Tsukuba
Institute of Livestock and Grassland Science, Naro
Abdel-Ghaffar, Alaa E.
Egypt, Benha
Faculty of Veterinary Medicine Benha University
Sosa, Gamal A.M.
Egypt, Benha
Faculty of Veterinary Medicine Benha University
Abou-El-Roos, Mahmoud E.A.
Egypt, Benha
Faculty of Veterinary Medicine Benha University
Nagai, Takashi
Japan, Tsukuba
Institute of Livestock and Grassland Science, Naro
Statistics
Citations: 169
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1002/mrd.21295
ISSN:
1040452X
e-ISSN:
10982795
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health