Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
A new cacao linkage map based on codominant markers: Development and integration of 201 new microsatellite markers
Theoretical and Applied Genetics, Volume 108, No. 6, Year 2004
Notification
URL copied to clipboard!
Description
A linkage map of cacao based on codominant markers has been constructed by integrating 201 new simple sequence repeats (SSR) developed in this study with a number of isoenzymes, restriction fragment length polymorphisms (RFLP), microsatellite markers and resistance and defence gene analogs (Rgenes-RFLP) previously mapped in cacao. A genomic library enriched for (GA)n and (CA)n was constructed, and 201 new microsatellite loci were mapped on 135 individuals from the same mapping population used to establish the first reference maps. This progeny resulted from a cross between two heterozygous cacao clones: an Upper-Amazon Forastero (UPA 402) and a Trinitario (UF 676). The new map contains 465 markers (268 SSRs, 176 RFLPs, five isoenzymes and 16 Rgenes-RFLP) arranged in ten linkage groups corresponding to the haploid chromosome number of cacao. Its length is 782.8 cM, with an average interval distance between markers of 1.7 cM. The new microsatellite markers were distributed throughout all linkage groups of the map, but their distribution was not random. The length of the map established with only SSRs was 769.6 cM, representing 94.8% of the total map. The current level of genome coverage is approximately one microsatellite every 3 cM. This new reference map provides a set of useful markers that is transferable across different mapping populations and will allow the identification and comparison of the most important regions involved in the variation of the traits of interest and the development of marker-assisted selection strategies. © Springer-Verlag 2004.
Authors & Co-Authors
Pugh, Tatiana
France, Paris
Cirad
Venezuela, Caracas
Universidad Central de Venezuela
Fouet, Olivier
France, Paris
Cirad
Risterucci, Ange Marie
France, Paris
Cirad
Brottier, Philippe
France, Evry
Genoscope - Centre National de Séquençage
Abouladze, M.
France, Paris
Cirad
Deletrez, C.
France, Paris
Cirad
Courtois, Brigitte
France, Paris
Cirad
Clément, Didier Pierre Louis
France, Paris
Cirad
Larmande, Pierre
France, Paris
Cirad
N'Goran, Jeanne Andi Kohi
Cote D'ivoire, Abidjan
Centre National de Recherche Agronomique Abidjan
Lanaud, Claire
France, Paris
Cirad
Statistics
Citations: 121
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1007/s00122-003-1533-4
ISSN:
00405752
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study