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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Direct contact of umbilical cord blood endothelial progenitors with living cardiac tissue is a requirement for vascular tube-like structures formation
Journal of Cellular and Molecular Medicine, Volume 15, No. 9, Year 2011
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Description
The umbilical cord blood derived endothelial progenitor cells (EPCs) contribute to vascular regeneration in experimental models of ischaemia. However, their ability to participate in cardiovascular tissue restoration has not been elucidated yet. We employed a novel coculture system to investigate whether human EPCs have the capacity to integrate into living and ischaemic cardiac tissue, and participate to neovascularization. EPCs were cocultured with either living or ischaemic murine embryonic ventricular slices, in the presence or absence of a pro-angiogenic growth factor cocktail consisting of VEGF, IGF-1, EGF and bFGF. Tracking of EPCs within the cocultures was performed by cell transfection with green fluorescent protein or by immunostaining performed with anti-human vWF, CD31, nuclei and mitochondria antibodies. EPCs generated vascular tube-like structures in direct contact with the living ventricular slices. Furthermore, the pro-angiogenic growth factor cocktail reduced significantly tubes formation. Coculture of EPCs with the living ventricular slices in a transwell system did not lead to vascular tube-like structures formation, demonstrating that the direct contact is necessary and that the soluble factors secreted by the living slices were not sufficient for their induction. No vascular tubes were formed when EPCs were cocultured with ischaemic ventricular slices, even in the presence of the pro-angiogenic cocktail. In conclusion, EPCs form vascular tube-like structures in contact with living cardiac tissue and the direct cell-to-cell interaction is a prerequisite for their induction. Understanding the cardiac niche and micro-environmental interactions that regulate EPCs integration and neovascularization are essential for applying these cells to cardiovascular regeneration. © 2011 The Authors Journal compilation © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD1.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD2.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD3.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD4.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD5.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD6.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD7.wmv
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD8.wmv
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD9.avi
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD10.wmv
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD11.wmv
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD12.avi
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD13.wmv
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD14.wmv
https://efashare.b-cdn.net/share/pmc/articles/PMC3918047/bin/jcmm0015-1914-SD15.tif
Authors & Co-Authors
Lupu, Marilena
Romania, Bucharest
Institute of Cellular Biology and Pathology Nicolae Simionescu
Khalil, Markus
Germany, Koln
Universität zu Köln
Iordache, Florin
Romania, Bucharest
Institute of Cellular Biology and Pathology Nicolae Simionescu
Andrei, Eugen
Romania, Bucharest
Institute of Cellular Biology and Pathology Nicolae Simionescu
Pfannkuche, Kurt
Germany, Koln
Universität zu Köln
Spitkovsky, Dimitry
Germany, Koln
Universität zu Köln
Baumgartner, Sven
Germany, Koln
Universität zu Köln
Rubach, Martin
Germany, Koln
Universität zu Köln
Abdelrazik, Heba
Egypt, Giza
Cairo University
Buzila, Cosmin
Romania, Bucharest
Institute of Cellular Biology and Pathology Nicolae Simionescu
Brockmeier, Konrad
Germany, Koln
Universität zu Köln
Simionescu, Maya
Romania, Bucharest
Institute of Cellular Biology and Pathology Nicolae Simionescu
Hescheler, Jürgen
Germany, Koln
Universität zu Köln
Maniu, Horia
Romania, Bucharest
Institute of Cellular Biology and Pathology Nicolae Simionescu
Statistics
Citations: 17
Authors: 14
Affiliations: 3
Identifiers
Doi:
10.1111/j.1582-4934.2010.01197.x
ISSN:
15821838
Research Areas
Health System And Policy
Noncommunicable Diseases